Cytokine Profile in Plasma Extracellular Vesicles of Parkinson's Disease and the Association with Cognitive Function.
Cytokine Profile in Plasma Extracellular Vesicles of Parkinson's Disease and the Association with Cognitive Function.
复制标题
DOI：
10.3390/cells10030604
复制
发表时间：
2021-03-09
期刊：
影响因子：
6
通讯作者：
Hong CT
中科院分区：
文献类型：
--
作者：
Chan L;Chung CC;Chen JH;Yu RC;Hong CT
血浆细胞外囊泡（EV）含有多种分子，包括细胞因子，可以反映细胞内状况并参与细胞间信号传导，从而成为帕金森病（PD）的生物标志物。炎症可能是帕金森病发生和进展的关键危险因素。本研究调查了血浆 EV 细胞因子作为 PD 生物标志物的作用。这项横断面研究招募了 113 名轻至中度 PD 患者和 48 名对照者。分离血浆EV，并评估细胞因子的水平，包括白细胞介素原（IL）-1β、IL-6、IL-10、肿瘤坏死因子（TNF）-α和转化生长因子（TGF）-β1。调整年龄和性别后，与对照组相比，PD 患者血浆 EV pro-IL-1β 和 TNF-α 水平显着升高。尽管 PD 患者血浆 EV 细胞因子与运动症状严重程度之间缺乏显着关联，但使用简易精神状态检查 (MMSE) 和蒙特利尔认知评估评估的认知功能障碍严重程度与血浆 EV pro-IL-1β、IL-6、IL-10 和 TNF-α 水平显着相关。这种关联是 PD 特异性的，在对照中未发现。此外，与没有认知缺陷的患者相比，患有 PD 认知缺陷的患者 (MMSE < 26) 表现出明显的 EV 细胞因子谱。这些发现支持了帕金森病发生和进展过程中炎症发病机制的概念，并表明血浆 EV 细胞因子将来可能作为帕金森病的生物标志物。
Plasma extracellular vesicles (EVs) containing various molecules, including cytokines, can reflect the intracellular condition and participate in cell-to-cell signaling, thus emerging as biomarkers for Parkinson’s disease (PD). Inflammation may be a crucial risk factor for PD development and progression. The present study investigated the role of plasma EV cytokines as the biomarkers of PD. This cross-sectional study recruited 113 patients with PD, with mild to moderate stage disease, and 48 controls. Plasma EVs were isolated, and the levels of cytokines, including pro-interleukin (IL)-1β, IL-6, IL-10, tumor necrosis factor (TNF)-α, and transforming growth factor (TGF)-β1, were evaluated. Patients with PD had significantly increased plasma EV pro-IL-1β and TNF-α levels compared with controls after adjustment for age and sex. Despite the lack of a significant association between plasma EV cytokines and motor symptom severity in patients with PD, cognitive dysfunction severity, assessed using the Mini-Mental State Examination (MMSE) and Montreal Cognitive Assessment, was significantly associated with plasma EV pro-IL-1β, IL-6, IL-10, and TNF-α levels. This association was PD specific and not found in controls. Furthermore, patients with PD cognitive deficit (MMSE < 26) exhibited a distinguished EV cytokine profile compared to those without cognitive deficit. The findings support the concept of inflammatory pathogenesis in the development and progression of PD and indicate that plasma EV cytokines may serve as PD biomarkers in future.
登录
查看更多内容
影响因子：
7.3
作者：
Rea IM;Gibson DS;McGilligan V;McNerlan SE;Alexander HD;Ross OA
通讯作者：
Ross OA
影响因子：
11
作者：
HUGHES, AJ;DANIEL, SE;LEES, AJ
通讯作者：
LEES, AJ
影响因子：
6.4
作者：
Konadu, Kateena Addae;Chu, Jane;Bond, Vincent C.
通讯作者：
Bond, Vincent C.
影响因子：
16
作者：
Lee, Jung-Hyun;Wittki, Sebastian;Baur, Andreas S.
通讯作者：
Baur, Andreas S.
影响因子：
4.6
作者：
Chung CC;Chan L;Bamodu OA;Hong CT;Chiu HW
通讯作者：
Chiu HW