Understanding of the importance of the spore coat structure and pigmentation in the Bacillus subtilis spore resistance to low-pressure plasma sterilization
Understanding of the importance of the spore coat structure and pigmentation in the Bacillus subtilis spore resistance to low-pressure plasma sterilization
复制标题
了解孢子衣结构和色素沉着在枯草芽孢杆菌孢子耐低压等离子体灭菌中的重要性
DOI：
10.1088/0022-3727/49/28/285401
复制
发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Moeller R
中科院分区：
文献类型：
--
作者：
Raguse M;Fiebrandt M;Denis B;Stapelmann K;Eichenberger P;Driks A;Eaton P;Awakowicz;Moeller R
低压等离子体在生物医学和国防方面的潜力已经得到了评估。等离子体的灭菌效果可以归因于几种活性剂，包括（V）UV辐射、带电粒子、自由基物质、中性和激发的原子和分子以及电场。以枯草芽孢杆菌孢子为生物指示剂和遗传模型系统，研究了低压等离子体去污对枯草芽孢杆菌孢子的杀灭效果。野生型孢子，孢子缺乏主要的保护涂层（内，外，和地壳），色素缺乏的孢子或孢子受损的外壳（在涂层组装的后期步骤）进行了系统的测试，其耐低压氩气，氢气和氧气等离子体与和没有混合物。我们表明，低压等离子体放电的氩气和氧气放电造成显着的物理损伤孢子表面结构可视化的原子力显微镜。孢子对低压等离子体的抵抗力主要取决于内部和外部孢子衣层以及孢子包裹的存在，外壳和孢子色素沉着的重要性较小或较低，而孢子灭活本身受到气体成分和操作设置的强烈影响。
Low-pressure plasmas have been evaluated for their potential in biomedical and defense purposes. The sterilizing effect of plasma can be attributed to several active agents, including (V) UV radiation, charged particles, radical species, neutral and excited atoms and molecules, and the electric field. Spores of Bacillus subtilis were used as a bioindicator and a genetic model system to study the sporicidal effects of low-pressure plasma decontamination. Wild-type spores, spores lacking the major protective coat layers (inner, outer, and crust), pigmentation-deficient spores or spore impaired in encasement (a late step in coat assembly) were systematically tested for their resistance to low-pressure argon, hydrogen, and oxygen plasmas with and without admixtures. We demonstrate that low-pressure plasma discharges of argon and oxygen discharges cause significant physical damage to spore surface structures as visualized by atomic force microscopy. Spore resistance to low-pressure plasma was primarily dependent on the presence of the inner, and outer spore coat layers as well as spore encasement, with minor or less importance of the crust and spore pigmentation, whereas spore inactivation itself was strongly influenced by the gas composition and operational settings.
登录
查看更多内容
通过大气中的直流放电对水和表面进行生物净化
DOI：
--
发表时间：
2012
期刊：
影响因子：
--
作者：
Z. Machala;B. Tarabová;Michal Pelach;Z. Sipoldova;K. Hensel;M. Janda;L. Šikurová
通讯作者：
L. Šikurová
DOI：
--
发表时间：
2012
期刊：
影响因子：
--
作者：
S. Lerouge
通讯作者：
S. Lerouge
影响因子：
3.7
作者：
Setlow, Peter
通讯作者：
Setlow, Peter
影响因子：
5.6
作者：
DONOVAN, W;ZHENG, L;LOSICK, R
通讯作者：
LOSICK, R
通过在单层中均匀分布枯草芽孢杆菌孢子来改进生物指标以评估强化孢子净化技术
影响因子：
4.4
作者：
Raguse M;Fiebrandt M;Stapelmann K;Madela K;Laue M;Lackmann JW;Thwaite JE;Setlow P;Awakowicz P;Moeller R
通讯作者：
Moeller R