Improvement of Biological Indicators by Uniformly Distributing Bacillus subtilis Spores in Monolayers To Evaluate Enhanced Spore Decontamination Technologies
Improvement of Biological Indicators by Uniformly Distributing Bacillus subtilis Spores in Monolayers To Evaluate Enhanced Spore Decontamination Technologies
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通过在单层中均匀分布枯草芽孢杆菌孢子来改进生物指标以评估强化孢子净化技术
DOI：
10.1128/aem.03934-15
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发表时间：
2016
影响因子：
4.4
通讯作者：
Moeller R
中科院分区：
文献类型：
--
作者：
Raguse M;Fiebrandt M;Stapelmann K;Madela K;Laue M;Lackmann JW;Thwaite JE;Setlow P;Awakowicz P;Moeller R
新型去污技术，包括冷低压等离子体和蓝光（400 nm），是传统表面去污方法的有前途的替代品。然而，对这种灭菌过程的评估标准化仍有待完成。芽孢杆菌属和土芽孢杆菌属的细菌内生孢子经常被用作无菌性的生物指示剂（BI）。确保标准化和可重复的BI用于可靠的测试程序是工业环境中的一个重要问题。在这项研究中，电驱动的喷雾沉积装置的开发，允许快速，重现性好，均匀的制备枯草芽孢杆菌168孢子单层的玻璃表面。详细介绍了该喷雾装置的结构设计和工作原理。通过扫描电子显微镜验证了每个样品高达5 × 107个孢子的孢子单层的可重复形成。表面灭活研究表明，单层孢子灭活UV-C（254 nm），低压氩等离子体（500 W，10 Pa，100标准立方厘米每分钟），和蓝光（400 nm）显着快于多层孢子。因此，我们成功地均匀制备了可重复的、高浓度的孢子单层，具有为各种非渗透性表面净化技术产生生物指示物的潜力。
Novel decontamination technologies, including cold low-pressure plasma and blue light (400 nm), are promising alternatives to conventional surface decontamination methods. However, the standardization of the assessment of such sterilization processes remains to be accomplished. Bacterial endospores of the genera Bacillus and Geobacillus are frequently used as biological indicators (BIs) of sterility. Ensuring standardized and reproducible BIs for reliable testing procedures is a significant problem in industrial settings. In this study, an electrically driven spray deposition device was developed, allowing fast, reproducible, and homogeneous preparation of Bacillus subtilis 168 spore monolayers on glass surfaces. A detailed description of the structural design as well as the operating principle of the spraying device is given. The reproducible formation of spore monolayers of up to 5 × 107spores per sample was verified by scanning electron microscopy. Surface inactivation studies revealed that monolayered spores were inactivated by UV-C (254 nm), low-pressure argon plasma (500 W, 10 Pa, 100 standard cubic cm per min), and blue light (400 nm) significantly faster than multilayered spores were. We have thus succeeded in the uniform preparation of reproducible, highly concentrated spore monolayers with the potential to generate BIs for a variety of nonpenetrating surface decontamination techniques.
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目前对气体等离子体灭菌微生物数据的错误解释
DOI：
--
发表时间：
2015
期刊：
影响因子：
--
作者：
H. Shintani
通讯作者：
H. Shintani
芽孢杆菌孢子气溶胶表面接种方法的开发
影响因子：
4.4
作者：
Sang Don D Lee;S. Ryan;E. Snyder
通讯作者：
E. Snyder
影响因子：
6.9
作者：
Humphreys, P. N.
通讯作者：
Humphreys, P. N.
环境储存相对湿度对生物指示剂抗性、活力和水分含量的影响
影响因子：
4.4
作者：
R. Reich;L. L. Morien
通讯作者：
L. L. Morien
无菌保证用生物指示剂性能变异的原因分析
影响因子：
--
作者：
H. Shintani;J. Akers
通讯作者：
J. Akers