Metabolic footprint of diabetes: a multiplatform metabolomics study in an epidemiological setting.
Metabolic footprint of diabetes: a multiplatform metabolomics study in an epidemiological setting.
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DOI：
10.1371/journal.pone.0013953
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发表时间：
2010-11-11
期刊：
影响因子：
3.7
通讯作者：
Illig T
中科院分区：
文献类型：
--
作者：
Suhre K;Meisinger C;Döring A;Altmaier E;Belcredi P;Gieger C;Chang D;Milburn MV;Gall WE;Weinberger KM;Mewes HW;Hrabé de Angelis M;Wichmann HE;Kronenberg F;Adamski J;Illig T
代谢组学是一个快速发展的领域，理想情况下，生物液体中所有内源性代谢产物的综合测量。然而，没有单一的分析技术涵盖整个人类代谢谱。在这里，我们提出了一个多平台研究的结果，在该研究中，我们调查了什么样的结果，目前可以在糖尿病研究领域时，结合代谢组学数据收集的一组互补的分析平台的流行病学研究的框架。从基于人群的KORA（奥格斯堡地区合作健康研究）研究的参与者中随机选择了40名自我报告的糖尿病患者和60名对照（男性，54岁以上），代表了德国普通人群的广泛表型样本。使用三种不同的技术，包括核磁共振和串联质谱法，在隔夜空腹血液中测定了超过420种独特小分子的浓度。已知的糖尿病生物标志物可以通过这种多代谢组学平台方法复制，包括糖代谢物（1，5-脱水葡萄糖醇），酮体（3-羟基丁酸酯）和支链氨基酸。在某些情况下，可以检测到糖尿病相关药物（吡格列酮、水杨酸）。我们的研究描绘了代谢组学在糖尿病研究中的潜力，通过鉴定一系列已知的和新的，与糖尿病相关的去调节的代谢物。关键观察结果包括与肾功能障碍（3-吲哚硫酸盐）、脂质代谢（甘油磷脂、游离脂肪酸）相关的代谢途径扰动以及与肠道微生物菌群（胆汁酸）的相互作用。我们的研究表明，代谢标志物有可能在一般人群的亚临床条件下检测糖尿病相关并发症。
Metabolomics is the rapidly evolving field of the comprehensive measurement of ideally all endogenous metabolites in a biological fluid. However, no single analytic technique covers the entire spectrum of the human metabolome. Here we present results from a multiplatform study, in which we investigate what kind of results can presently be obtained in the field of diabetes research when combining metabolomics data collected on a complementary set of analytical platforms in the framework of an epidemiological study. 40 individuals with self-reported diabetes and 60 controls (male, over 54 years) were randomly selected from the participants of the population-based KORA (Cooperative Health Research in the Region of Augsburg) study, representing an extensively phenotyped sample of the general German population. Concentrations of over 420 unique small molecules were determined in overnight-fasting blood using three different techniques, covering nuclear magnetic resonance and tandem mass spectrometry. Known biomarkers of diabetes could be replicated by this multiple metabolomic platform approach, including sugar metabolites (1,5-anhydroglucoitol), ketone bodies (3-hydroxybutyrate), and branched chain amino acids. In some cases, diabetes-related medication can be detected (pioglitazone, salicylic acid). Our study depicts the promising potential of metabolomics in diabetes research by identification of a series of known and also novel, deregulated metabolites that associate with diabetes. Key observations include perturbations of metabolic pathways linked to kidney dysfunction (3-indoxyl sulfate), lipid metabolism (glycerophospholipids, free fatty acids), and interaction with the gut microflora (bile acids). Our study suggests that metabolic markers hold the potential to detect diabetes-related complications already under sub-clinical conditions in the general population.
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影响因子：
7.7
作者：
BORGHI, L;LUGARI, R;GNUDI, A
通讯作者：
GNUDI, A
DOI：
10.1002/dmr.5610040803
发表时间：
1988-12-01
期刊：
DIABETES-METABOLISM REVIEWS
影响因子：
--
作者：
GOLAY, A;FELBER, JP;FERRANNINI, E
通讯作者：
FERRANNINI, E
影响因子：
2.1
作者：
GLANVILLE, NT;ANDERSON, GH
通讯作者：
ANDERSON, GH
影响因子：
5.2
作者：
Altmaier, Elisabeth;Kastenmueller, Gabi;Suhre, Karsten
通讯作者：
Suhre, Karsten
DOI：
10.1073/pnas.0705685105
发表时间：
2008-02-05
影响因子：
11.1
作者：
Assfalg, Michael;Bertini, Ivano;Spraul, Manfred
通讯作者：
Spraul, Manfred