Specific lectin biomarkers for isolation of human pluripotent stem cells identified through array-based glycomic analysis.
Specific lectin biomarkers for isolation of human pluripotent stem cells identified through array-based glycomic analysis.
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作者：
快速和可靠的方法分离人多能干细胞（hPSC）群体是迫切需要的基础研究和基于细胞的治疗应用的质量控制。使用凝集素阵列，我们分析了从26个hPSC样品和22个分化的细胞样品中提取的糖蛋白，并鉴定了一小组具有独特结合特征的凝集素，其足以将hPSC与各种非多能细胞类型区分开。这些特异性生物标志物被检查的所有12个人胚胎干细胞和14个人诱导多能干细胞样品所共有，无论实验室来源、培养条件、重编程的体细胞类型或所使用的重编程方法如何。我们证明了特异性凝集素结合的实际应用，通过用凝集素介导的染色检测分化细胞群内的hPSC，然后用荧光显微镜和流式细胞术，以及通过使用凝集素介导的细胞分离从混合细胞群中富集和净化活的hPSC。全球基因表达分析表明，多能性相关的特异性岩藻糖基转移酶和唾液酸转移酶的差异表达，这可能是这些差异的蛋白质糖基化和凝集素结合的基础。总之，我们的结果表明，蛋白质糖基化在多能和非多能细胞之间有很大的不同，并证明凝集素可用作生物标志物来监测干细胞群体中的多能性和从混合细胞群体中去除活的hPSC。
Rapid and dependable methods for isolating human pluripotent stem cell (hPSC) populations are urgently needed for quality control in basic research and in cell-based therapy applications. Using lectin arrays, we analyzed glycoproteins extracted from 26 hPSC samples and 22 differentiated cell samples, and identified a small group of lectins with distinctive binding signatures that were sufficient to distinguish hPSCs from a variety of non-pluripotent cell types. These specific biomarkers were shared by all the 12 human embryonic stem cell and the 14 human induced pluripotent stem cell samples examined, regardless of the laboratory of origin, the culture conditions, the somatic cell type reprogrammed, or the reprogramming method used. We demonstrated a practical application of specific lectin binding by detecting hPSCs within a differentiated cell population with lectin-mediated staining followed by fluorescence microscopy and flow cytometry, and by enriching and purging viable hPSCs from mixed cell populations using lectin-mediated cell separation. Global gene expression analysis showed pluripotency-associated differential expression of specific fucosyltransferases and sialyltransferases, which may underlie these differences in protein glycosylation and lectin binding. Taken together, our results show that protein glycosylation differs considerably between pluripotent and non-pluripotent cells, and demonstrate that lectins may be used as biomarkers to monitor pluripotency in stem cell populations and for removal of viable hPSCs from mixed cell populations.
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影响因子：
64.5
作者：
Moody, AM;Chui, D;Reinherz, EL
通讯作者：
Reinherz, EL
DOI：
10.1007/bf01534848
发表时间：
1984-01-01
期刊：
SOMATIC CELL AND MOLECULAR GENETICS
影响因子：
--
作者：
DRABER, P;STANLEY, P
通讯作者：
STANLEY, P
影响因子：
16.2
作者：
LANDMESSER, L;DAHM, L;RUTISHAUSER, U
通讯作者：
RUTISHAUSER, U
DOI：
10.1016/s0022-3476(99)70281-7
发表时间：
1999-06
期刊：
The Journal of pediatrics
影响因子：
--
作者：
Marquardt T;Brune T;Lühn K;Zimmer KP;Körner C;Fabritz L;van der Werft N;Vormoor J;Freeze HH;Louwen F;Biermann B;Harms E;von Figura K;Vestweber D;Koch HG
通讯作者：
Koch HG
影响因子：
48
作者：
Kuno, A;Uchiyama, N;Hirabayashi, J
通讯作者：
Hirabayashi, J