NMR spectroscopy analysis reveals differential metabolic responses in arabidopsis roots and leaves treated with a cytokinesis inhibitor.
NMR spectroscopy analysis reveals differential metabolic responses in arabidopsis roots and leaves treated with a cytokinesis inhibitor.
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DOI：
10.1371/journal.pone.0241627
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发表时间：
2020
期刊：
影响因子：
3.7
通讯作者：
Drakakaki G
中科院分区：
文献类型：
--
作者：
Wilkop TE;Wang M;Heringer A;Singh J;Zakharov F;Krishnan VV;Drakakaki G
在植物细胞质分裂中，新形成的细胞板进化成新的细胞壁，将分裂细胞的细胞质分隔开。在我们早期的化学基因组学研究中，我们鉴定并表征了小分子内糖素-7，它特异性地抑制细胞板上的胼胝质沉积，阻止拟南芥后期的细胞分裂。内啡肽-7已经成为一个非常有价值的工具来解剖这一重要的植物过程。为了进一步了解其作用方式和细胞分裂抑制对植物整体反应的影响，我们通过核磁共振波谱（NMR）代谢组学方法研究了内sidin-7的作用。在本研究中，研究人员分析了拟南芥叶片和根组织在不同生长阶段和不同暴露水平下的代谢组学特征。结果显示了叶片和根系代谢谱的变化以及内糖素-7处理对这些代谢组的影响。统计分析表明，内啡肽-7治疗的效果比发育影响更显著。内sidin-7诱导的代谢谱表明在中枢代谢途径中对细胞分裂抑制的补偿。本研究进一步表明，长期处理内啡肽-7可能通过改变激素调节，改变拟南芥幼苗的初级代谢。激素通路的变化可能反映了植物的反应，补偿了细胞分裂的停滞，这反过来又导致了全球代谢物的调节。所呈现的核磁共振光谱数据可通过代谢组学工作台获得，为科学界提供参考资源。
In plant cytokinesis, de novo formation of a cell plate evolving into the new cell wall partitions the cytoplasm of the dividing cell. In our earlier chemical genomics studies, we identified and characterized the small molecule endosidin-7, that specifically inhibits callose deposition at the cell plate, arresting late-stage cytokinesis in arabidopsis. Endosidin-7 has emerged as a very valuable tool for dissecting this essential plant process. To gain insights regarding its mode of action and the effects of cytokinesis inhibition on the overall plant response, we investigated the effect of endosidin-7 through a nuclear magnetic resonance spectroscopy (NMR) metabolomics approach. In this case study, metabolomics profiles of arabidopsis leaf and root tissues were analyzed at different growth stages and endosidin-7 exposure levels. The results show leaf and root-specific metabolic profile changes and the effects of endosidin-7 treatment on these metabolomes. Statistical analyses indicated that the effect of endosidin-7 treatment was more significant than the developmental impact. The endosidin-7 induced metabolic profiles suggest compensations for cytokinesis inhibition in central metabolism pathways. This study further shows that long-term treatment of endosidin-7 profoundly changes, likely via alteration of hormonal regulation, the primary metabolism of arabidopsis seedlings. Hormonal pathway-changes are likely reflecting the plant’s responses, compensating for the arrested cell division, which in turn are leading to global metabolite modulation. The presented NMR spectral data are made available through the Metabolomics Workbench, providing a reference resource for the scientific community.
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影响因子：
6.9
作者：
Brandt, S;Kloska, S;Kehr, J
通讯作者：
Kehr, J
影响因子：
3.7
作者：
Augustijn, Dieuwertje;van Tol, Niels;Alia, A.
通讯作者：
Alia, A.
拟南芥中细胞分裂素调节基因。
影响因子：
5.6
作者：
Brenner WG;Ramireddy E;Heyl A;Schmülling T
通讯作者：
Schmülling T
DOI：
10.1199/tab.0137
发表时间：
2010-01-01
期刊：
The arabidopsis book
影响因子：
--
作者：
Binder, Stefan
通讯作者：
Binder, Stefan
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y