Optical molecular imaging for systems biology: from molecule to organism.
Optical molecular imaging for systems biology: from molecule to organism.
复制标题
系统生物学的光学分子成像：从分子到有机体
DOI：
10.1007/s00216-006-0541-z
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发表时间：
2006-10
影响因子：
4.3
通讯作者：
Liu, Bi-Feng
中科院分区：
文献类型：
--
作者：
Du, Wei;Wang, Ying;Luo, Qingming;Liu, Bi-Feng
开发能够在系统水平上探测生物系统的高效分析方法是一项重要的任务，这是为了满足新兴的系统生物学领域的要求。光学分子成像技术是研究生物体内特定生物分子时空动态及其相互作用的一种真实的有力工具。本文综述了近年来OMI的研究进展，如分子探针的开发，使成像更明亮，更稳定，信息量更大（例如，FP和半导体纳米晶体，也称为量子点），提供更高分辨率和更大组织渗透的成像方法的开发，以及用于测量从分子到生物体水平的生物事件的应用，包括基因表达，蛋白质和亚细胞区室定位，蛋白质活化和相互作用，以及低质量分子动力学。这些进展在生物科学领域具有重要意义，也可应用于疾病诊断和药物筛选。OMI系统生物学的进一步发展也提出了建议。
The development of highly efficient analytical methods capable of probing biological systems at system level is an important task that is required in order to meet the requirements of the emerging field of systems biology. Optical molecular imaging (OMI) is a very powerful tool for studying the temporal and spatial dynamics of specific biomolecules and their interactions in real time in vivo. In this article, recent advances in OMI are reviewed extensively, such as the development of molecular probes that make imaging brighter, more stable and more informative (e.g., FPs and semiconductor nanocrystals, also referred to as quantum dots), the development of imaging approaches that provide higher resolution and greater tissue penetration, and applications for measuring biological events from molecule to organism level, including gene expression, protein and subcellular compartment localization, protein activation and interaction, and low-mass molecule dynamics. These advances are of great significance in the field of biological science and could also be applied to disease diagnosis and pharmaceutical screening. Further developments in OMI for systems biology are also proposed.
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DOI：
10.1073/pnas.152463399
发表时间：
2002-10-01
影响因子：
11.1
作者：
Åkerman, ME;Chan, WCW;Ruoslahti, E
通讯作者：
Ruoslahti, E
影响因子：
3.9
作者：
Chiang, CF;Okou, DT;Williams, MNV
通讯作者：
Williams, MNV
影响因子：
11.6
作者：
Dhonukshe, P;Gadella, TWJ
通讯作者：
Gadella, TWJ
影响因子：
3.4
作者：
Caspers, PJ;Lucassen, GW;Puppels, GJ
通讯作者：
Puppels, GJ
影响因子：
3.4
作者：
Ataka, K;Pieribone, VA
通讯作者：
Pieribone, VA