The OsHAPL1-DTH8-Hd1 complex functions as the transcription regulator to repress heading date in rice.
The OsHAPL1-DTH8-Hd1 complex functions as the transcription regulator to repress heading date in rice.
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OsHAPL1-DTH8-Hd1 复合物作为转录调节因子抑制水稻抽穗期
DOI：
10.1093/jxb/erw468
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发表时间：
2017-01-01
影响因子：
6.9
通讯作者：
Wan J
中科院分区：
文献类型：
--
作者：
Zhu S;Wang J;Cai M;Zhang H;Wu F;Xu Y;Li C;Cheng Z;Zhang X;Guo X;Sheng P;Wu M;Wang J;Lei C;Wang J;Zhao Z;Wu C;Wang H;Wan J
开花时间是决定作物品种适应性的重要性状。一种新的抽穗期调节因子OsHAPL1与DTH8-HD1相互作用，负调控水稻的开花时间。抽穗期是影响作物产量的重要农艺性状。在水稻中已经发现了许多与抽穗期相关的基因，也已经建立了一个复杂的、初步的调控遗传网络，但对蛋白质调控网络却知之甚少。我们已经确定了一种新的抽穗期调节因子，血红素激活蛋白样蛋白1(OsHAPL1)，它可以在长日照条件下抑制开花。OsHAPL1是一种核定位蛋白，在叶片中以有节奏的方式高表达。OsHAPL1可以与8号染色体上的抽穗天数(DTH8)相互作用，而DTH8在体外和体内都与抽穗日期1(HD1)相互作用。OsHAPL1在大肠杆菌中与DTH8和HD1形成复合体。OsHAPL1、DTH8和HD1在物理上与HAP复合体相互作用，也与酵母(酿酒酵母)中的一般转录因子相互作用。进一步的研究表明，OsHAPL1在抽穗期1号(EHD1)期间抑制成花基因和开花基因T1(RFT1)和Hd3a的表达。我们认为OsHAPL1作为一种转录调控因子，与DTH8、HD1、HAP复合体和一般转录因子一起调节靶基因的表达，然后通过Ehd1影响Hd3a和RFT1的表达，从而影响抽穗期。
Flowering time is an important trait for determining the adaptability of a crop cultivar. A novel heading date regulator, OsHAPL1, interacts with DTH8-Hd1 to negatively regulate flowering time in rice. Heading date is an important agronomic trait related to crop yield. Many genes related to heading date have already been identified in rice (Oryza sativa), and a complicated, preliminary regulatory genetic network has also already been established, but the protein regulatory network is poorly understood. We have identified a novel heading date regulator, Heme Activator Protein like 1 (OsHAPL1), which inhibits flowering under long-day conditions. OsHAPL1 is a nuclear-localized protein that is highly expressed in leaves in a rhythmic manner. OsHAPL1 can physically interact with Days To Heading on chromosome 8 (DTH8), which physically interacts with Heading date 1 (Hd1) both in vitro and in vivo. OsHAPL1 forms a complex with DTH8 and Hd1 in Escherichia coli. OsHAPL1, DTH8, and Hd1 physically interact with the HAP complex, and also with general transcription factors in yeast (Saccharomyces cerevisiae). Further studies showed that OsHAPL1 represses the expression of the florigen genes and FLOWERING LOCUS T 1 (RFT1) and Hd3a through Early heading date 1 (Ehd1). We propose that OsHAPL1 functions as a transcriptional regulator and, together with DTH8, Hd1, the HAP complex, and general transcription factors, regulates the expression of target genes and then affects heading date by influencing the expression of Hd3a and RFT1 through Ehd1.
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影响因子：
4.5
作者：
Gao H;Zheng XM;Fei G;Chen J;Jin M;Ren Y;Wu W;Zhou K;Sheng P;Zhou F;Jiang L;Wang J;Zhang X;Guo X;Wang JL;Cheng Z;Wu C;Wang H;Wan JM
通讯作者：
Wan JM
影响因子：
5.1
作者：
Bart R;Chern M;Park CJ;Bartley L;Ronald PC
通讯作者：
Ronald PC
影响因子：
11.6
作者：
Hanano, Shigeru;Goto, Koji
通讯作者：
Goto, Koji
影响因子：
7.4
作者：
Kim, Song Lim;Lee, Shinyoung;An, Gynheung
通讯作者：
An, Gynheung
影响因子：
64.8
作者：
Hayama, R;Yokoi, S;Shimamoto, K
通讯作者：
Shimamoto, K