Reply to evolutionary flux of canonical microRNAs and mirtrons in Drosophila.
Reply to evolutionary flux of canonical microRNAs and mirtrons in Drosophila.
复制标题
作者：
It has been known for some time that there are many weakly expressed and fast-evolving microRNAs (miRNAs) 1–6. After analyzing more than 100,000 reads of small RNAs obtained from Drosophila heads, we suggested that most of these miRNAs were born and then died with the evolutionary dynamics of neutrally evolving sequences7. The birth and death rates of miRNAs were estimated to be about 12 and 11.7 genes per Myr, respectively, resulting in a fairly modest net gain of only 0.3 genes per Myr. Berezikov et al. revised the estimate of net gain to be about 1 gene per Myr but did not provide an estimate of the birth and death rates separately8. Instead, they argued that our estimates for both the birth and death rates were too high by claiming that many of the miRNAs in our analysis were not miRNAs at all but were merely degraded products of mRNAs.Their main concern was that many of the newly born miRNAs in our observation were singletons. In their expanded data, many of these singletons were missing and some were accompanied by sequences of similar length in the same hairpin, both of which suggested RNA degradation. Being mindful of the limitation of low coverage in our study7, we have collected 18 million small RNA reads in Drosophila heads by sequencing of oligonucleotide ligation and detection (SOLiD) sequencing. We analyzed only reads that appeared at least 50 times in the arm of a hairpin and for which the accurate processing rate of the 5′ end of the miRNAs
登录
查看更多内容
影响因子：
3.7
作者：
Fahlgren N;Howell MD;Kasschau KD;Chapman EJ;Sullivan CM;Cumbie JS;Givan SA;Law TF;Grant SR;Dangl JL;Carrington JC
通讯作者：
Carrington JC
影响因子：
30.8
作者：
Bentwich, I;Avniel, A;Bentwich, Z
通讯作者：
Bentwich, Z
影响因子：
14.9
作者：
Kloosterman WP;Steiner FA;Berezikov E;de Bruijn E;van de Belt J;Verheul M;Cuppen E;Plasterk RH
通讯作者：
Plasterk RH
影响因子：
10.7
作者：
Liang, Han;Li, Wen-Hsiung
通讯作者：
Li, Wen-Hsiung
影响因子：
10.7
作者：
Das, Sabyasachi
通讯作者：
Das, Sabyasachi