Human enteroviruses associated with and without diarrhea in Thailand between 2010 and 2016.
Human enteroviruses associated with and without diarrhea in Thailand between 2010 and 2016.
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DOI：
10.1371/journal.pone.0182078
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Poovorawan Y
中科院分区：
文献类型：
--
作者：
Chansaenroj J;Tuanthap S;Thanusuwannasak T;Duang-In A;Klinfueng S;Thaneskongtong N;Vutithanachot V;Vongpunsawad S;Poovorawan Y
与病毒感染相关的非细菌性急性胃肠炎（AGE）影响生活在发展中国家的个人，特别是儿童。为了研究某些人类肠道病毒（EV）的脱落是否在病因不明的AGE个体的粪便中比没有AGE症状的个体更频繁地检测到，我们测试了2010年1月至2016年12月期间从2，692名腹泻个体中收集的粪便样本。通过逆转录聚合酶链反应（RT-PCR）检测样本中的轮状病毒、诺如病毒和EV，并通过PCR检测腺病毒。EV阳性样本进行测序和系统发育分析，以确定EV的种类和类型。研究结果与1，310份来自被诊断患有手足口病（HFMD）的非AGE个体的粪便样本中发现的EV进行了比较。在AGE中鉴定的大多数病毒包括人轮状病毒（22.7%）、诺如病毒（11.4%）和腺病毒（9.3%），我们鉴定的EV（6.2%）主要属于B、C和鼻病毒。相反，92%以上的EV没有AGE症状，属于A种。虽然AGE症状通常不归因于EV感染，但至少在3.4%的AGE病例中，EV与病因不明的腹泻有关。虽然CV-A6最有可能在手足口病患者的粪便中发现，但鼻病毒A和C是与AGE相关的两种最常见的EV种类。阐明群体特异性EV感染的疾病与非AGE将有助于识别，临床管理，并在社区EV感染的监测。
Non-bacterial acute gastroenteritis (AGE) associated with virus infection affects individuals living in developing countries, especially children. To investigate whether shedding of certain human enterovirus (EV) is more frequently detected in the stool of individuals with AGE of unknown etiology than individuals without AGE symptoms, we tested fecal samples collected from 2,692 individuals with diarrhea between January 2010 and December 2016. Samples were tested for rotavirus, norovirus, and EV by reverse-transcription polymerase chain reaction (RT-PCR) and adenovirus by PCR. EV-positive samples were subjected to sequencing and phylogenetic analysis to identify EV species and types. Findings were compared to EV found in 1,310 fecal samples from individuals without AGE who were diagnosed with hand, foot, and mouth disease (HFMD). While the majority of viruses identified in AGE consisted of human rotavirus (22.7%), norovirus (11.4%) and adenovirus (9.3%), we identified EV (6.2%) belonging mainly to species B, C, and rhinovirus. In contrast, >92% of EV found without AGE symptoms belonged to species A. Although AGE symptoms are not often attributed to EV infection, EV was associated with diarrhea of unknown etiology at least in 3.4% of AGE cases. While CV-A6 was most likely to be found in stools of HFMD patients, rhinovirus A and C were the two most common EV species associated with AGE. Elucidating group-specific EV infection in diseases with and without AGE will be useful in assisting identification, clinical management, and the surveillance of EV infection in the community.
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影响因子：
3.7
作者：
Holtz, Lori R.;Cao, Song;Zhao, Guoyan;Bauer, Irma K.;Denno, Donna M.;Klein, Eileen J.;Antonio, Martin;Stine, O. Cohn;Snelling, Thomas L.;Kirkwood, Carl D.;Wang, David
通讯作者：
Wang, David
影响因子：
168.9
作者：
Lozano, Rafael;Naghavi, Mohsen;Murray, Christopher J. L.
通讯作者：
Murray, Christopher J. L.
影响因子：
12.7
作者：
Harvala, H.;McIntyre, C. L.;Simmonds, P.
通讯作者：
Simmonds, P.
影响因子：
168.9
作者：
Kotloff, Karen L.;Nataro, James P.;Levine, Myron M.
通讯作者：
Levine, Myron M.
DOI：
10.1016/b978-0-444-53488-0.00018-3
发表时间：
2014-01-01
期刊：
NEUROVIROLOGY
影响因子：
--
作者：
Jubelt, Burk;Lipton, Howard L.
通讯作者：
Lipton, Howard L.