Morphological effects of somatostatin on rat somatotrophs previously activated by growth hormone-releasing factor
Morphological effects of somatostatin on rat somatotrophs previously activated by growth hormone-releasing factor
复制标题
生长抑素对先前被生长激素释放因子激活的大鼠生长激素细胞的形态学影响
DOI：
10.1007/bf00318663
复制
发表时间：
1990
影响因子：
3.6
通讯作者：
H. Ishikawa
中科院分区：
文献类型：
--
作者：
O. Shimada;H. Tosaka;H. Ishikawa
摘要为了阐明生长抑素在体内抑制大鼠垂体前叶生长激素（GH）分泌的作用，进行了相关的形态学和生理学分析。透射电子显微镜结合免疫金标记显示，注射 GH 释放因子 (GRF) 后 2 至 10 分钟固定的生长激素细胞中含有 GH 的胞吐分泌颗粒数量增加。注射GRF还诱导高尔基体池、许多包膜囊泡和多囊泡体中出现免疫阳性物质。在整个细胞质中更频繁地观察到微管，特别是在高尔基体区域内和附近。注射生长抑素(SRIF)后2分钟和10分钟，先前受GRF刺激的生长激素细胞中的胞吐数和血浆中放射免疫测定的GH的量均明显减少。 GRF 引起的质膜 (PM) 波动迅速消失，质膜正下方的颗粒数量显着减少。注射 SRIF 后，在颗粒和质膜之间的空间中经常观察到平行的微丝束。 SRIF 并未导致高尔基体区域免疫阳性物质、包被囊泡和多泡体数量的明显减少，也没有导致微管分布的任何显着变化。因此，SRIF 似乎主要在质膜水平上抑制激素释放，可能是通过改变微丝的分布来实现的。
SummaryCorrelative morphological and physiological analysis was carried out in order to clarify the role of somatostatin in the inhibition of the secretion of growth hormone (GH) from somatotrophs of the rat anterior pituitary gland in vivo. Transmission electron microscopy combined with immunogold labelling showed an increased number of exocytotic GH-containing secretory granules in somatotrophs fixed between 2 and 10 min after injection of GH-releasing factor (GRF). Injection of GRF also induced the appearance of immunopositive material in cisternae of the Golgi apparatus, many coated vesicles and multivesicular bodies. Microtubules were observed more frequently throughout the cytoplasm, particularly in and near the Golgi region. At 2 and 10 min after injection of somatostatin (SRIF), both the number of exocytotic figures in the somatotrophs previously stimulated by GRF and the amount of radioimmunoassayable GH in the plasma were clearly decreased. Undulation of the plasma membrane (PM) induced by GRF rapidly disappeared, and the number of granules just beneath the plasma membrane was significantly reduced. After injection of SRIF, parallel bundles of microfilaments were often observed in the space between the granules and the plasma membrane. SRIF did not cause a noticeable decrease in the amount of immunopositive material, coated vesicles and multivesicular bodies in the Golgi areas or any significant changes in the distribution of microtubules. SRIF therefore appears to inhibit hormone release mainly at the level of the plasma membrane, probably through changes in the distribution of microfilaments.
登录
查看更多内容
影响因子：
56.9
作者：
GUILLEMIN, R;BRAZEAU, P;WEHRENBERG, WB
通讯作者：
WEHRENBERG, WB
生长激素释放因子，生长激素释放因子，刺激麻醉大鼠生长激素的分泌。
DOI：
10.1016/0006-291x(82)91732-6
发表时间：
1982
影响因子：
3.1
作者：
Wehrenberg,WB;Ling,N;Brazeau,P;Esch,F;Böhlen,P;Baird,A;Ying,S;Guillemin,R
通讯作者：
Guillemin,R
大鼠生长激素（rGH）释放因子和生长抑素对分散垂体细胞中 rGH 释放和合成的影响。
影响因子：
4.8
作者：
Fukata,J;Diamond,DJ;Martin,JB
通讯作者：
Martin,JB
分散的正常雄性大鼠垂体细胞中 45Ca2 动员与 GH 释放因子刺激生长激素 (GH) 释放的关联。
影响因子：
4.8
作者：
Login,IS;Judd,AM;MacLeod,RM
通讯作者：
MacLeod,RM
刺激和抑制分泌的结构相关性：灌注大鼠垂体中生长激素的电子显微镜观察。
影响因子：
4.1
作者：
Stachura,ME;Costoff,A;Tyler,JM
通讯作者：
Tyler,JM