Impact of FADS gene variation and dietary fatty acid exposure on biochemical and anthropomorphic phenotypes in a Hispanic/Latino cohort.
Impact of FADS gene variation and dietary fatty acid exposure on biochemical and anthropomorphic phenotypes in a Hispanic/Latino cohort.
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DOI：
10.3389/fnut.2023.1111624
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发表时间：
2023
影响因子：
5
通讯作者：
Chilton, Floyd H.
中科院分区：
文献类型：
--
作者：
Sergeant, Susan;Keith, Brian A.;Seeds, Michael C.;Legins, Jimaree A.;Young, Caroline B.;Vitolins, Mara Z.;Chilton, Floyd H.
关键词：
多不饱和脂肪酸（PUFA）和高度不饱和脂肪酸（HUFA）的合成产物及其信号代谢产物在免疫、炎症和脑发育/功能中起着至关重要的作用。脂肪酸去饱和酶（FADS）基因簇内变异体的频率差异影响HUFA水平、其生物活性产物和许多生理表型。关于这种遗传变异对西班牙裔/拉丁裔人口健康的影响，仍然存在根本问题。来自135名参与者（83.7%女性）的数据和生物标本（血浆，红细胞，血沉棕黄层衍生的DNA）用于评估饮食PUFA水平，FADS单倍型标记SNP，rs 174537和西班牙裔/拉丁裔人群在血浆和RBC中产生HUFA的能力之间的关系，以及其对拟人表型的潜在影响。队列的饮食习惯显示，参与者的饮食中含有高比例（9.3 ± 0.2，平均值± SEM）的亚油酸（n−6）与α-亚麻酸（n−3），并且还含有极低水平的n−3 HUFA（二十碳五烯酸，EPA和二十二碳六烯酸，DHA），这两种特征都是现代西方饮食的特征。与非洲裔和欧洲裔美国人队列相比，该西班牙裔/拉丁裔队列中TT rs 174537基因型的频率高度富集（53%的受试者），并与较低的循环HUFA水平密切相关。例如，TT基因型中花生四烯酸（ARA：20：4，n-6）和EPA（20：5，n-3）的血浆水平分别比GG基因型低37%和23%。通过代谢产物与前体的比率确定的HUFA生物合成效率高度依赖于（p < 0.0001）rs 174537基因型（GG > GT > TT）循环n-6和n-3 HUFA。相比之下，该人群的RBC Omega-3指数（EPA + DHA）极低（2.89 ± 1.65，平均值±标准差），与rs 174537基因型无关。重要的是，rs 174537基因型也与女性身高相关，TT基因型参与者比GG + GT参与者矮4.5 cm（p = 0.0001）。总之，这项研究表明，饮食PUFA + HUFA × FADS基因相互作用使大部分（>50%）西班牙裔/拉丁裔人群处于循环和细胞水平n−3 HUFA缺乏的高风险中。
Polyunsaturated fatty acids (PUFA) and highly unsaturated fatty acid (HUFA) synthetic products and their signaling metabolites play vital roles in immunity, inflammation, and brain development/function. Frequency differences of variants within the fatty acid desaturase (FADS) gene cluster affect levels of HUFAs, their biologically active products, and numerous physiological phenotypes. Fundamental questions remain regarding the impact of this genetic variation on the health of Hispanic/Latino populations. Data and biospecimens (plasma, red blood cells, buffy coat-derived DNA) from 135 participants (83.7% female) were used to assess the relationship(s) between dietary PUFA levels, a FADS haplotype tagging SNP, rs174537, and the capacity of Hispanic/Latino populations to generate HUFAs in plasma and RBC as well as its potential impact on anthropomorphic phenotypes. The dietary habits of the cohort showed that participant diets contained a high ratio (9.3 ± 0.2, mean ± SEM) of linoleic acid (n−6) to alpha-linolenic acid (n−3) and also contained extremely low levels of n−3 HUFAs (eicosapentaenoic acid, EPA and docosahexaenoic acid, DHA), both features of the Modern Western Diet. Compared to African and European American cohorts, the frequency of the TT rs174537 genotype was highly enriched (53% of subjects) in this Hispanic/Latino cohort and was strongly associated with lower circulating HUFA levels. For example, plasma levels of arachidonic acid (ARA: 20:4, n−6) and EPA (20:5, n−3) were 37% and 23%, respectively, lower in the TT versus the GG genotype. HUFA biosynthetic efficiency, as determined by metabolic product to precursor ratios, was highly dependent (p < 0.0001) on the rs174537 genotype (GG > GT > TT) for both circulating n−6 and n−3 HUFAs. In contrast, the RBC Omega-3 Index (EPA + DHA) was extremely low (2.89 ± 1.65, mean ± sd) in this population and independent of rs174537 genotype. Importantly, the rs174537 genotype was also related to female height with TT genotype participants being 4.5 cm shorter (p = 0.0001) than the GG + GT participants. Taken together, this study illustrates that dietary PUFA + HUFA × FADS gene- interactions place a large proportion (>50%) of Hispanic/Latino populations at high risk of a deficiency in both circulating and cellular levels of n−3 HUFAs.
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DOI：
10.1093/ajcn/nqy089
发表时间：
2018-08-01
期刊：
The American journal of clinical nutrition
影响因子：
--
作者：
Brenna JT;Plourde M;Stark KD;Jones PJ;Lin YH
通讯作者：
Lin YH
影响因子：
5.8
作者：
Harris, William S.
通讯作者：
Harris, William S.
影响因子：
5
作者：
Kipnis, V;Subar, AF;Carroll, RJ
通讯作者：
Carroll, RJ
影响因子：
16.6
作者：
Fadason, Tayaza;Schierding, William;O'Sullivan, Justin M.
通讯作者：
O'Sullivan, Justin M.
影响因子：
5.1
作者：
Harris, WS;von Schacky, C
通讯作者：
von Schacky, C