Best practices for the design, laboratory analysis, and reporting of trials involving fatty acids.
Best practices for the design, laboratory analysis, and reporting of trials involving fatty acids.
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DOI：
10.1093/ajcn/nqy089
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发表时间：
2018-08-01
期刊：
影响因子：
--
通讯作者：
Lin YH
中科院分区：
文献类型：
--
作者：
Brenna JT;Plourde M;Stark KD;Jones PJ;Lin YH
脂肪酸是人体代谢和健康中研究最多的营养素之一。内源性脂肪酸状态通过多种机制在生命周期的各个阶段影响健康和疾病。尽管广泛的兴趣，试图通过荟萃分析和系统评价总结多项研究的结果，解决类似的脂肪酸相关的结果已经令人失望，主要是因为研究设计，采样，实验室和数据分析的异质性。我们的目的是为脂肪酸临床营养和医学研究推荐最佳实践。研究设计中的关键问题包括明智地选择用于脂肪酸分析的内源性样本池，考虑相关生理状态、干预和/或观察的持续时间，考虑特定脂肪酸动力学以将摄入量和内源性浓度联系起来，以及根据已知脂肪酸范围对结果进行解释。关键实验室考虑因素包括适当的样品储存、使用通过已发表的验证研究已知适用的样品制备方法、建立适当脂肪酸鉴别的详细报告或方法，以及定量分析，包括差异响应的校准、质量控制程序和报告最小脂肪酸组的数据，以实现全面解释。我们提出了一个脂肪酸最佳实践的建议清单，以促进设计，审查和评估的研究，旨在提高研究的重现性。
Fatty acids are among the most studied nutrients in human metabolism and health. Endogenous fatty acid status influences health and disease via multiple mechanisms at all stages of the life cycle. Despite widespread interest, attempts to summarize the results of multiple studies addressing similar fatty acid–related outcomes via meta-analyses and systematic reviews have been disappointing, largely because of heterogeneity in study design, sampling, and laboratory and data analyses. Our purpose is to recommend best practices for fatty acid clinical nutrition and medical studies. Key issues in study design include judicious choice of sampled endogenous pools for fatty acid analysis, considering relevant physiologic state, duration of intervention and/or observation, consideration of specific fatty acid dynamics to link intake and endogenous concentrations, and interpretation of results with respect to known fatty acid ranges. Key laboratory considerations include proper sample storage, use of sample preparation methods known to be fit-for-purpose via published validation studies, detailed reporting or methods to establish proper fatty acid identification, and quantitative analysis, including calibration of differential response, quality control procedures, and reporting of data on a minimal set of fatty acids to enable comprehensive interpretation. We present a checklist of recommendations for fatty acid best practices to facilitate design, review, and evaluation of studies with the intention of improving study reproducibility.
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DOI：
10.1016/j.chroma.2008.10.006
发表时间：
2008-11-28
期刊：
Journal of chromatography. A
影响因子：
--
作者：
Araujo P;Nguyen TT;Frøyland L;Wang J;Kang JX
通讯作者：
Kang JX
影响因子：
5.4
作者：
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Kris-Etherton PM
DOI：
10.1007/s005990070007
发表时间：
2000-12-01
期刊：
INTERNATIONAL JOURNAL OF CLINICAL & LABORATORY RESEARCH
影响因子：
--
作者：
Di Marino, L;Maffettene, A;Panico, S
通讯作者：
Panico, S
影响因子：
1.9
作者：
Bailey-Hall, Eileen;Nelson, Edward B.;Ryan, Alan S.
通讯作者：
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影响因子：
1.2
作者：
Fisk, Helena L.;West, Annette L.;Calder, Philip C.
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Calder, Philip C.