An instant beverage rich in nutrients and secondary metabolites manufactured from stems and leaves of Panax notoginseng.

An instant beverage rich in nutrients and secondary metabolites manufactured from stems and leaves of Panax notoginseng.
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以三七茎叶为原料,富含营养物质和次生代谢产物的速溶饮料

DOI:
10.3389/fnut.2022.1058639
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发表时间:
2022
影响因子:
5
通讯作者:
Zhao, Ming
Zhao, Ming
中科院分区:
农林科学2区
文献类型:
--
作者:
Liang, Zhengwei;Liu, Kunyi;Li, Ruoyu;Ma, Baiping;Zheng, Wei;Yang, Shengchao;Zhang, Guanghui;Zhao, Yinhe;Chen, Junwen;Zhao, Ming

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三七是五加科植物三七的干燥根,是我国著名的传统中药。三七茎叶含有丰富的次生代谢产物和营养成分,是一种公认的食品资源,但其开发利用还有待进一步研究。通过响应面设计优化,以21倍量的48.50%乙醇提取39 h,将该提取重复两次;提取液使用真空旋转蒸发器在45°C下浓缩至1/3体积,然后在110°C下喷雾干燥,从SLPN制备SLPN-速溶饮料。结果表明,该饮料含有14种氨基酸、10种矿物质元素、15种维生素,其中含有43种三萜皂苷类化合物,采用UPLC-MS法对SLPN-速溶饮料中的黄酮类成分进行了鉴定,结果表明SLPN-速溶饮料的提取率为37.89 ± 0.02%。主要营养成分为甘氨酸(2.10 ± 0.63 mg/g)、组氨酸(1.23 ± 0.07 mg/g)、α-VE(18.89 ± 1.87 μg/g)、β-VE(17.53 ± 1.98 μg/g)、钾(49.26 ± 2.70 mg/g)、钙(6.73 ± 0.27 mg/g)。SLPN速溶饮料中总皂苷含量为403.05 ± 34.98 mg/g,主要为三七皂苷,含量为227 ± 2.02 mg/g。儿茶素和γ-氨基丁酸的含量分别为24.57 ± 0.21 mg/g和7.50 ± 1.85 mg/g。SLPN-速溶饮料对羟自由基(OH-)、超氧阴离子自由基(O2-)、DPPH自由基和2,2 ′-连氮双(3-乙基苯并噻唑啉-6-磺酸盐)(ABTS+)自由基的浓度分别为0.1954、0.2314、0.4083和0.3874 mg/mL。我们优化了一种分析方法,用于深入分析新授权的食品资源SLPN。同时,以SLPN为原料,制备了一种具有抗氧化活性、富含营养成分和次生代谢产物的速溶饮料,提高了SLPN的利用率。
Radix Notoginseng, one of the most famous Chinese traditional medicines, is the dried root of Panax notoginseng (Araliaceae). Stems and leaves of P. notoginseng (SLPN) are rich in secondary metabolites and nutrients, and authorized as a food resource, however, its utilization needs further research. A SLPN-instant beverage was manufactured from SLPN through optimization by response surface design with 21-fold of 48.50% ethanol for 39 h, and this extraction was repeated twice; the extraction solution was concentrated to 1/3 volume using a vacuum rotatory evaporator at 45°C, and then spray dried at 110°C. Nutritional components including 14 amino acids, ten mineral elements, 15 vitamins were detected in the SLPN-instant beverage; forty-three triterpenoid saponins, e.g., ginsenoside La, ginsenoside Rb3, notoginsenoside R1, and two flavonoid glycosides, as well as dencichine were identified by UPLC-MS. The extraction rate of SLPN-instant beverage was 37.89 ± 0.02%. The majority nutrients were Gly (2.10 ± 0.63 mg/g), His (1.23 ± 0.07 mg/g), α-VE (18.89 ± 1.87 μg/g), β-VE (17.53 ± 1.98 μg/g), potassium (49.26 ± 2.70 mg/g), calcium (6.73 ± 0.27 mg/g). The total saponin of the SLPN-instant beverage was 403.05 ± 34.98 mg/g, majority was notoginsenoside Fd and with contents of 227 ± 2.02 mg/g. In addition, catechin and γ-aminobutyric acid were detected with levels of 24.57 ± 0.21 mg/g and 7.50 ± 1.85 mg/g, respectively. The SLPN-instant beverage showed good antioxidant activities with half maximal inhibitory concentration (IC50) for scavenging hydroxyl (OH–) radicals, superoxide anion (O2–) radicals, 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonate) (ABTS+) radicals were 0.1954, 0.2314, 0.4083, and 0.3874 mg/mL, respectively. We optimized an analytical method for in depth analysis of the newly authorized food resource SLPN. Together, an instant beverage with antioxidant activity, rich in nutrients and secondary metabolites, was manufactured from SLPN, which may improve the utilization of SLPN.
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