Image-Based Elastography of Heterochromatin and Euchromatin Domains in the Deforming Cell Nucleus.
Image-Based Elastography of Heterochromatin and Euchromatin Domains in the Deforming Cell Nucleus.
复制标题
变形细胞核中异染色质和常染色质结构域的基于图像的弹性成像。
DOI：
10.1002/smll.202006109
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发表时间：
2021-03
期刊：
影响因子：
--
通讯作者：
Neu CP
中科院分区：
文献类型：
--
作者：
Ghosh S;Cuevas VC;Seelbinder B;Neu CP
Chromatin of the eukaryotic cell nucleus comprises of microscopically dense heterochromatin and loose euchromatin domains, each with distinct transcriptional ability and roles in cellular mechanotransduction. While recent methods have been developed to characterize the mechanics of nucleus, measurement of intranuclear mechanics remains largely unknown. Here, we describe the development of nuclear elastography, which combines microscopic imaging and computational modeling to quantify the relative elasticity of the heterochromatin and euchromatin domains. Using contracting murine embryonic cardiomyocytes, nuclear elastography reveals that the heterochromatin is almost four times stiffer than the euchromatin at peak deformation. The relative elasticity between the two domains changes rapidly during the active deformation of the cardiomyocyte in the normal physiological condition but progresses more slowly in cells cultured in a mechanically stiff environment, although the relative stiffness at peak deformation does not change. Further, we found that the disruption of the KASH domain of the LINC complex compromises the intranuclear elasticity distribution resulting in elastically similar heterochromatin and euchromatin. These results provide insight into the elastography dynamics of heterochromatin and euchromatin domains and provide a non-invasive framework to further investigate the mechanobiological function of subcellular and subnuclear domains limited only by the spatiotemporal resolution of the acquired images. Optical microscopy, image analysis, and computational modeling are used in combination to investigate the intranuclear elastography. Relative stiffness of euchromatin and heterochromatin domains are quantified non-invasively. Compared to a soft mechanical environment, on a stiff substrate the dynamics of nuclear elastography is altered. Nuclear envelope protein disruption affects the relative stiffness of the intranuclear space.
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DOI：
10.1186/1755-1536-5-15
发表时间：
2012-09-03
期刊：
Fibrogenesis & tissue repair
影响因子：
--
作者：
Fan D;Takawale A;Lee J;Kassiri Z
通讯作者：
Kassiri Z
影响因子：
17.1
作者：
Guerrero, Carlos R.;Garcia, Pablo D.;Garcia, Ricardo
通讯作者：
Garcia, Ricardo
影响因子：
8.8
作者：
Ghosh S;Seelbinder B;Henderson JT;Watts RD;Scott AK;Veress AI;Neu CP
通讯作者：
Neu CP
亚细胞结构的生物力学通过非侵入性布里鲁因显微镜。
影响因子：
4.6
作者：
Antonacci G;Braakman S
通讯作者：
Braakman S
影响因子：
41.2
作者：
Dingal PC;Bradshaw AM;Cho S;Raab M;Buxboim A;Swift J;Discher DE
通讯作者：
Discher DE