High Sensitivity Singlet Oxygen Luminescence Sensor Using Computational Spectroscopy and Solid-State Detector.
High Sensitivity Singlet Oxygen Luminescence Sensor Using Computational Spectroscopy and Solid-State Detector.
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使用计算光谱和固态检测器的高灵敏度单线发光传感器。
DOI：
10.3390/diagnostics13223431
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发表时间：
2023-11-12
期刊：
影响因子：
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本文提出了一种高灵敏度测量光动力疗法（PDT）和紫外线（UV）照射皮肤过程中产生的单线态氧发光的技术。高测量灵敏度是通过使用计算光谱（CS）的方法，提供了改进的光子检测效率相比，光谱滤波方法。固态InGaAs光电二极管被用作CS检测器，与光电倍增管相比，这显著降低了系统成本并提高了鲁棒性。光谱分辨率使得能够高精度地确定和减去光敏剂荧光基线，而不需要时间选通。这允许高灵敏度检测由连续波光源产生的单线态氧发光发射，所述连续波光源例如太阳模拟器源和PDT诊所中常用的那些光源。该技术的价值在体内和体外实验中得到了证明，这些实验显示了测量的单线态氧与PDT治疗功效和皮肤上的照明强度的相关性。这些结果表明，该技术作为剂量计的潜在用途，以指导PDT治疗，并作为一种分析工具，支持开发改进的防晒产品，预防皮肤癌。
This paper presents a technique for high sensitivity measurement of singlet oxygen luminescence generated during photodynamic therapy (PDT) and ultraviolet (UV) irradiation on skin. The high measurement sensitivity is achieved by using a computational spectroscopy (CS) approach that provides improved photon detection efficiency compared to spectral filtering methodology. A solid-state InGaAs photodiode is used as the CS detector, which significantly reduces system cost and improves robustness compared to photomultiplier tubes. The spectral resolution enables high-accuracy determination and subtraction of photosensitizer fluorescence baseline without the need for time-gating. This allows for high sensitivity detection of singlet oxygen luminescence emission generated by continuous wave light sources, such as solar simulator sources and those commonly used in PDT clinics. The value of the technology is demonstrated during in vivo and ex vivo experiments that show the correlation of measured singlet oxygen with PDT treatment efficacy and the illumination intensity on the skin. These results demonstrate the potential use of the technology as a dosimeter to guide PDT treatment and as an analytical tool supporting the development of improved sunscreen products for skin cancer prevention.
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DOI：
10.3390/molecules28104085
发表时间：
2023-05-14
期刊：
Molecules (Basel, Switzerland)
影响因子：
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作者：
Fujii J;Soma Y;Matsuda Y
通讯作者：
Matsuda Y
影响因子：
3.4
作者：
Jarvi, Mark T.;Patterson, Michael S.;Wilson, Brian C.
通讯作者：
Wilson, Brian C.
影响因子：
3.1
作者：
Jimenez-Banzo, Ana;Ragas, Xavier;Nonell, Santi
通讯作者：
Nonell, Santi
影响因子：
3.3
作者：
Dysart, JS;Singh, G;Patterson, MS
通讯作者：
Patterson, MS
影响因子：
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作者：
Buckel, TBH;Goldstein, AM;Tucker, MA
通讯作者：
Tucker, MA