Analyzing mosquito (Diptera: culicidae) diversity in Pakistan by DNA barcoding.
Analyzing mosquito (Diptera: culicidae) diversity in Pakistan by DNA barcoding.
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DOI：
10.1371/journal.pone.0097268
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Mirza MS
中科院分区：
文献类型：
--
作者：
Ashfaq M;Hebert PD;Mirza JH;Khan AM;Zafar Y;Mirza MS
尽管蚊子是重要的疾病媒介，但巴基斯坦对蚊子的生物多样性知之甚少。最近的登革热流行揭示了需要更详细地了解该地区蚊子物种的多样性和分布。DNA条形码提高了蚊子清单的准确性，因为许多物种之间的形态差异是微妙的，导致错误识别。线粒体COI基因条形码区域的序列变异被用来识别蚊子物种，揭示遗传多样性，并绘制登革热媒介物种在巴基斯坦的分布图。2010-2013年期间，对旁遮普和开伯尔-普赫图赫瓦省491个地点的1684只蚊子进行了分析，发现32种蚊子以致倦库蚊为主（占收集总数的61%）。伊蚊属（Stegomyia）占标本的15%，由6个分类群和两种登革热媒介物种Ae. albopictus和Ae.埃及种，占优势，分布广泛。按蚊占安捕获量的6%。subpictus主导。同种标本的条形码序列差异范围为0- 2.4%，而同属物种显示2.3-17.8%的差异。全球疾病载体单倍型分析显示存在多种单倍型，尽管在大多数国家，每个登革热载体物种的单一单倍型占主导地位。Ae的地理分布。埃及伊蚊和埃及伊蚊。白纹伊蚊则以后者占优势，在农村和城市都有分布。作为巴基斯坦蚊子的第一个基于DNA的分析，这项研究已经开始构建该地区蚊子的条形码参考库。不同物种的遗传多样性水平不同。由于其能够区分物种，即使是那些具有细微形态差异的物种，DNA条形码有助于准确追踪病媒种群。
Although they are important disease vectors mosquito biodiversity in Pakistan is poorly known. Recent epidemics of dengue fever have revealed the need for more detailed understanding of the diversity and distributions of mosquito species in this region. DNA barcoding improves the accuracy of mosquito inventories because morphological differences between many species are subtle, leading to misidentifications. Sequence variation in the barcode region of the mitochondrial COI gene was used to identify mosquito species, reveal genetic diversity, and map the distribution of the dengue-vector species in Pakistan. Analysis of 1684 mosquitoes from 491 sites in Punjab and Khyber Pakhtunkhwa during 2010–2013 revealed 32 species with the assemblage dominated by Culex quinquefasciatus (61% of the collection). The genus Aedes (Stegomyia) comprised 15% of the specimens, and was represented by six taxa with the two dengue vector species, Ae. albopictus and Ae. aegypti, dominant and broadly distributed. Anopheles made up another 6% of the catch with An. subpictus dominating. Barcode sequence divergence in conspecific specimens ranged from 0–2.4%, while congeneric species showed from 2.3–17.8% divergence. A global haplotype analysis of disease-vectors showed the presence of multiple haplotypes, although a single haplotype of each dengue-vector species was dominant in most countries. Geographic distribution of Ae. aegypti and Ae. albopictus showed the later species was dominant and found in both rural and urban environments. As the first DNA-based analysis of mosquitoes in Pakistan, this study has begun the construction of a barcode reference library for the mosquitoes of this region. Levels of genetic diversity varied among species. Because of its capacity to differentiate species, even those with subtle morphological differences, DNA barcoding aids accurate tracking of vector populations.
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影响因子：
2.1
作者：
Braks, MAH;Honório, NA;Lounibos, LP
通讯作者：
Lounibos, LP
影响因子：
3.3
作者：
Anderson, JR;Schneider, JR;Severson, DW
通讯作者：
Severson, DW
DOI：
10.1098/rsbl.2003.0025
发表时间：
2003-08-07
影响因子：
4.7
作者：
Hebert, PDN;Ratnasingham, S;deWaard, JR
通讯作者：
deWaard, JR
影响因子：
56.9
作者：
Bossuyt, F;Meegaskumbura, M;Milinkovitch, MC
通讯作者：
Milinkovitch, MC
影响因子：
2.1
作者：
Delatte, H.;Dehecq, J. S.;Fontenille, D.
通讯作者：
Fontenille, D.