Differential Gel Electrophoresis and Transgenic Mitochondrial Calcium Reporters Demonstrate Spatiotemporal Filtering in Calcium Control of Mitochondria*
Differential Gel Electrophoresis and Transgenic Mitochondrial Calcium Reporters Demonstrate Spatiotemporal Filtering in Calcium Control of Mitochondria*
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差异凝胶电泳和转基因线粒体钙报告基因展示了线粒体钙控制中的时空过滤*
DOI：
10.1074/jbc.m603002200
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发表时间：
2006
影响因子：
4.8
通讯作者：
J. Dow
中科院分区：
文献类型：
--
作者：
Selim Terhzaz;T. Southall;K. Lilley;L. Kean;A. Allan;S. Davies;J. Dow
线粒体必须调整其细胞内位置和代谢，以平衡其输出以满足细胞的需要。在这里，我们通过时间序列差异凝胶电泳的蛋白质组学技术表明，果蝇肾小管神经内分泌刺激的主要结果是线粒体基质的广泛重塑。通过培养发光和荧光线粒体钙报告基因的果蝇，我们发现线粒体钙跟踪细胞质钙的缓慢变化（几分钟），而不是快速变化（<1秒），这导致线粒体膜极化增加和细胞ATP水平升高。选择性v -ATP酶抑制剂巴菲霉素进一步提高了ATP水平，表明顶质膜v -ATP酶是ATP的主要消耗者。线粒体钙受体阻滞剂Ru360可消除线粒体钙信号和ATP的增加。通过线粒体钙成像和电位传感染料JC-1，发现主细胞的顶端线粒体对神经肽信号有选择性响应。由于最终目标是顶质膜上的v - atp酶，线粒体的这种选择性激活显然是适应性的。这些结果强调了分化的器官型细胞中钙信号的动态性和时空异质性，并为v - atp酶的神经内分泌控制提供了新的模型。
Mitochondria must adjust both their intracellular location and their metabolism in order to balance their output to the needs of the cell. Here we show by the proteomic technique of time series difference gel electrophoresis that a major result of neuroendocrine stimulation of the Drosophila renal tubule is an extensive remodeling of the mitochondrial matrix. By generating Drosophila that were transgenic for both luminescent and fluorescent mitochondrial calcium reporters, it was shown that mitochondrial calcium tracked the slow (minutes) but not the rapid (<1 s) changes in cytoplasmic calcium and that this resulted in both increased mitochondrial membrane polarization and elevated cellular ATP levels. The selective V-ATPase inhibitor, bafilomycin, further enhanced ATP levels, suggesting that the apical plasma membrane V-ATPase is a major consumer of ATP. Both the mitochondrial calcium signal and the increase in ATP were abolished by the mitochondrial calcium uniporter blocker Ru360. By using both mitochondrial calcium imaging and the potential sensing dye JC-1, the apical mitochondria of principal cells were found to be selectively responsive to neuropeptide signaling. As the ultimate target is the V-ATPase in the apical plasma membrane, this selective activation of mitochondria is clearly adaptive. The results highlight the dynamic nature and both spatial and temporal heterogeneity of calcium signaling possible in differentiated, organotypic cells and provide a new model for neuroendocrine control of V-ATPase.
影响因子：
2.9
作者：
Hopper, RK;Carroll, S;Balaban, RS
通讯作者：
Balaban, RS
DOI：
10.1073/pnas.88.9.3671
发表时间：
1991-05-01
影响因子：
11.1
作者：
SMILEY, ST;REERS, M;CHEN, LB
通讯作者：
CHEN, LB
DOI：
10.1073/pnas.85.21.7972
发表时间：
1988-11-01
影响因子：
11.1
作者：
BOWMAN, EJ;SIEBERS, A;ALTENDORF, K
通讯作者：
ALTENDORF, K