Bottlenecks in erucic acid accumulation in genetically engineered ultrahigh erucic acid Crambe abyssinica.
Bottlenecks in erucic acid accumulation in genetically engineered ultrahigh erucic acid Crambe abyssinica.
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DOI：
10.1111/pbi.12128
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发表时间：
2014-02
影响因子：
13.8
通讯作者：
Zhu LH
中科院分区：
文献类型：
--
作者：
Guan R;Lager I;Li X;Stymne S;Zhu LH
芥酸是一种有价值的工业脂肪酸，具有多种用途。如今，这种酸的主要生产者是高芥子油菜 (Brassica napus) 和芥菜 (Brassica juncea)，其种子油中含有 45%–50% 的芥酸。海甘蓝 (Crambe abyssinica) 是这种酸的替代生产者，有前景，因为它的油中含有 55%–60% 的芥酸。通过对三个基因进行基因改造 (GM)，我们之前已将海甘蓝籽油中的芥酸含量提高到 71% (68 mol%)。在这项研究中，我们进一步研究了正在开发的转基因海甘蓝种子与野生型（Wt）海甘蓝、油菜籽和红花（Carthamustinctorius）相比油生物合成的不同方面。我们发现海甘蓝种子的磷脂酰胆碱-二酰甘油相互转化率非常低，这表明它是油生物合成过程中芥酸被限制在膜脂中的主要原因。我们进一步表明，转基因海甘蓝种子的种子发育速度比野生甘蓝种子慢，并且在开花后 (DAF) 的前 20 天内油脂积累速度也较慢。尽管在转基因种子发育的早期阶段芥酸积累量较低，但在 27 至 50 DAF 之间积累的所有脂肪酸中，近 86 mol% 是芥酸，此时总油的 40% 被沉积。讨论了转基因海甘蓝种子发育早期阶段芥酸积累的可能瓶颈。
Erucic acid is a valuable industrial fatty acid with many applications. The main producers of this acid are today high erucic rapeseed (Brassica napus) and mustard (Brassica juncea), which have 45%–50% of erucic acid in their seed oils. Crambe abyssinica is an alternative promising producer of this acid as it has 55%–60% of erucic acid in its oil. Through genetic modification (GM) of three genes, we have previously increased the level of erucic acid to 71% (68 mol%) in Crambe seed oil. In this study, we further investigated different aspects of oil biosynthesis in the developing GM Crambe seeds in comparison with wild-type (Wt) Crambe, rapeseed and safflower (Carthamus tinctorius). We show that Crambe seeds have very low phosphatidylcholine-diacylglycerol interconversion, suggesting it to be the main reason why erucic acid is limited in the membrane lipids during oil biosynthesis. We further show that GM Crambe seeds have slower seed development than Wt, accompanied by slower oil accumulation during the first 20 days after flowering (DAF). Despite low accumulation of erucic acid during early stages of GM seed development, nearly 86 mol% of all fatty acids accumulated between 27 and 50 DAF was erucic acid, when 40% of the total oil is laid down. Likely bottlenecks in the accumulation of erucic acid during early stages of GM Crambe seed development are discussed.
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