Oxidized extracellular DNA as a stress signal in human cells.
Oxidized extracellular DNA as a stress signal in human cells.
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DOI：
10.1155/2013/649747
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发表时间：
2013
影响因子：
--
通讯作者：
Veiko NN
中科院分区：
文献类型：
--
作者：
Ermakov AV;Konkova MS;Kostyuk SV;Izevskaya VL;Baranova A;Veiko NN
术语“无细胞DNA”（cfDNA）最近被创造出来，用于描述血浆/血清中的DNA片段，而存在于体外细胞培养基中的DNA被称为细胞外DNA （ecDNA）。在氧化应激条件下，细胞DNA的氧化修饰水平和细胞死亡率增加。死亡的细胞释放出受损的DNA，从而将氧化的DNA片段贡献给cfDNA/ecDNA池。氧化的无细胞DNA可以作为一种应激信号，促进辐射诱导的旁观者效应。有证据表明TLR9可能是氧化DNA传感器的候选者。暴露于氧化的ecDNA刺激活性氧（ROS）的合成，引发适应性反应，包括核内同源位点的转位，肌动蛋白的聚合和应激纤维的形成，以及核糖体基因表达的激活，NF-E2相关因子-2 （NRF2）的核易位，进而介导II期解毒和抗氧化酶的诱导。综上所述，氧化DNA是一种应激信号，是对培养细胞氧化应激的反应，也可能是对人体氧化应激的反应；特别是，它可能有助于局部照射治疗的全身外显效应。
The term “cell-free DNA” (cfDNA) was recently coined for DNA fragments from plasma/serum, while DNA present in in vitro cell culture media is known as extracellular DNA (ecDNA). Under oxidative stress conditions, the levels of oxidative modification of cellular DNA and the rate of cell death increase. Dying cells release their damaged DNA, thus, contributing oxidized DNA fragments to the pool of cfDNA/ecDNA. Oxidized cell-free DNA could serve as a stress signal that promotes irradiation-induced bystander effect. Evidence points to TLR9 as a possible candidate for oxidized DNA sensor. An exposure to oxidized ecDNA stimulates a synthesis of reactive oxygen species (ROS) that evokes an adaptive response that includes transposition of the homologous loci within the nucleus, polymerization and the formation of the stress fibers of the actin, as well as activation of the ribosomal gene expression, and nuclear translocation of NF-E2 related factor-2 (NRF2) that, in turn, mediates induction of phase II detoxifying and antioxidant enzymes. In conclusion, the oxidized DNA is a stress signal released in response to oxidative stress in the cultured cells and, possibly, in the human body; in particular, it might contribute to systemic abscopal effects of localized irradiation treatments.
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影响因子：
13.5
作者：
Bamboat, Zubin M.;Balachandran, Vinod P.;Ocuin, Lee M.;Obaid, Hebroon;Plitas, George;DeMatteo, Ronald P.
通讯作者：
DeMatteo, Ronald P.
影响因子：
--
作者：
Bloomer, Richard J.;Fisher-Wellman, Kelsey H.
通讯作者：
Fisher-Wellman, Kelsey H.
影响因子：
0.7
作者：
Efremova, L. V.;Alekseeva, A. Yu.;Veiko, N. N.
通讯作者：
Veiko, N. N.
影响因子：
3.3
作者：
Cooke, MS;Herbert, KE;Lunec, J
通讯作者：
Lunec, J
DOI：
10.1016/s0027-5107(02)00010-6
发表时间：
2002-04-25
影响因子：
2.3
作者：
Ballarini, F;Biaggi, M;Sapora, O
通讯作者：
Sapora, O