Evaluation of the ecotoxicity of sediments from Yangtze river estuary and contribution of priority PAHs to ah receptor--mediated activities.
Evaluation of the ecotoxicity of sediments from Yangtze river estuary and contribution of priority PAHs to ah receptor--mediated activities.
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长江口沉积物的生态毒性评价及优先PAHs对Ah受体介导活性的贡献
DOI：
10.1371/journal.pone.0104748
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Wu L
中科院分区：
文献类型：
--
作者：
Liu L;Chen L;Shao Y;Zhang L;Floehr T;Xiao H;Yan Y;Eichbaum K;Hollert H;Wu L
在这项研究中，通过体外生物测定来评估长江口沉积物的生态毒理潜力。用中性红滞留法和7-乙氧基间苯二酚-O-脱乙基酶活性测定了底物提取物对虹鱼肝细胞的细胞毒性和芳烃受体(AhR)介导的毒性。与其他水系沉积物相比，长江口沉积物的细胞毒性和AhR介导的活性处于低水平到中等水平。然而，与欧洲主要河流莱茵河相比，长江的泄水量大约是莱茵河的14倍。因此，长江的绝对污染传质可能会对河口和东中国海的环境质量造成不利影响。应用效果导向分析来确定导致高二恶英活性的物质。为了确定对整个提取物的二恶英样效价有贡献的未知物质，我们用开柱层析对粗提物进行了分级。从每个沉积物粗提物中分离出非极性烷烃组分(F1)、弱极性和中极性组分(F2)和高极性物质(F3)。F2的二恶英样活性最高。根据化学毒性当量浓度(TEQs)的质量平衡计算结果，我们的结论是，优先多环芳烃在粗提物中生物毒性当量浓度的比例很低，在1%到10%之间。应该进行进一步的研究，以确定未知污染物。
In this study, in vitro bioassays were performed to assess the ecotoxicological potential of sediments from Yangtze River estuary. The cytotoxicity and aryl hydrocarbon receptor (AhR)-mediated toxicity of sediment extracts with rainbow trout (Oncorhynchus mykiss) liver cells were determined by neutral red retention and 7-ethoxyresorufin-O-deethylase assays. The cytotoxicity and AhR-mediated activity of sediments from the Yangtze River estuary ranged from low level to moderate level compared with the ecotoxicity of sediments from other river systems. However, Yangtze River releases approximately 14 times greater water discharge compared with Rhine, a major river in Europe. Thus, the absolute pollution mass transfer of Yangtze River may be detrimental to the environmental quality of estuary and East China Sea. Effect-directed analysis was applied to identify substances causing high dioxin-like activities. To identify unknown substances contributing to dioxin-like potencies of whole extracts, we fractionated crude extracts by open column chromatography. Non-polar paraffinic components (F1), weakly and moderately polar components (F2), and highly polar substances (F3) were separated from each crude extract of sediments. F2 showed the highest dioxin-like activities. Based on the results of mass balance calculation of chemical toxic equivalent concentrations (TEQs), our conclusion is that priority polycyclic aromatic hydrocarbons indicated a low portion of bio-TEQs ranging from 1% to 10% of crude extracts. Further studies should be conducted to identify unknown pollutants.
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影响因子：
5.8
作者：
Gustavsson, LK;Klee, N;Engwall, M
通讯作者：
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