Identification of New Epididymal Luminal Fluid Proteins Involved in Sperm Maturation in Infertile Rats Treated by Dutasteride Using iTRAQ.
Identification of New Epididymal Luminal Fluid Proteins Involved in Sperm Maturation in Infertile Rats Treated by Dutasteride Using iTRAQ.
复制标题
使用 iTRAQ 鉴定度他雄胺治疗的不育大鼠中参与精子成熟的新附睾管腔流体蛋白
DOI：
10.3390/molecules21050602
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发表时间：
2016-05-11
期刊：
影响因子：
--
通讯作者：
Zhu Y
中科院分区：
文献类型：
--
作者：
Xie SW;Li GT;Qu LJ;Cao Y;Wang Q;Zhou JY;Zhong RH;Guo XJ;Zhu Y
背景：精子在通过附睾管腔的过程中成熟并获得受精能力。本研究通过双5α-还原酶抑制剂杜他雄胺在不育大鼠体内鉴定出参与精子成熟的新的附睾管内液蛋白，以期为新的避孕药和不孕症治疗提供潜在的附睾靶点。方法：雄性大鼠用杜他雄胺治疗28 d。采用等压标记相对绝对定量（iTRAQ）技术，观察了不育和正常大鼠附睾腔液中蛋白的表达谱。通过酶联免疫吸附试验验证了蛋白质组数据的可信度。结果：共检测到1045种蛋白，其中23种蛋白在不育大鼠和正常大鼠中表达谱不同。7个蛋白下调，16个蛋白上调。在被杜他雄胺显著下调的7个附睾腔液蛋白中，有3个β-防御素（Defb2、Defb18和Defb39）可能是参与附睾精子成熟和男性生育的关键蛋白。结论：我们首次报道了杜他雄胺对大鼠附睾腔液蛋白表达谱的影响，这一结果为男性避孕和不育治疗提供了一些新的附睾靶点。
Background: Spermatozoa become mature and acquire fertilizing capacity during their passage through the epididymal lumen. In this study, we identified new epididymal luminal fluid proteins involved in sperm maturation in infertile rats by dutasteride, a dual 5α-reductase inhibitor, in order to provide potential epididymal targets for new contraceptives and infertility treatment. Methods: Male rats were treated with dutasteride for 28 consecutive days. We observed the protein expression profiles in the epididymal luminal fluids in infertile and normal rats using isobaric tags for relative and absolute quantitation (iTRAQ) technique. The confidence of proteome data was validated by enzyme-linked immunosorbent assays. Results: 1045 proteins were tested, and 23 of them presented different expression profiling in the infertile and normal rats. The seven proteins were down-regulated, and 16 proteins were up-regulated. Among the seven proteins which were significantly down-regulated by dutasteride in the epididymal luminal fluids, there were three β-defensins (Defb2, Defb18 and Defb39), which maybe the key proteins involved in epididymal sperm maturation and male fertility. Conclusions: We report for the first time that dutasteride influences the protein expression profiling in the epididymal luminal fluids of rats, and this result provides some new epididymal targets for male contraception and infertility therapy.
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DOI：
10.1186/1477-7827-11-59
发表时间：
2013-07-01
期刊：
Reproductive biology and endocrinology : RB&E
影响因子：
--
作者：
Pujianto DA;Loanda E;Sari P;Midoen YH;Soeharso P
通讯作者：
Soeharso P
影响因子：
3.6
作者：
Seachrist, Darcie D.;Johnson, Emhonta;Keri, Ruth A.
通讯作者：
Keri, Ruth A.
影响因子：
4.8
作者：
Hamzeh, Mahsa;Robaire, Bernard
通讯作者：
Robaire, Bernard
影响因子：
48
作者：
Wisniewski, Jacek R.;Zougman, Alexandre;Mann, Matthias
通讯作者：
Mann, Matthias
影响因子：
--
作者：
Robaire, Bernard;Hamzeh, Mahsa
通讯作者：
Hamzeh, Mahsa