CyclinD1 and p57(kip2) as biomarkers in differentiation, metastasis and prognosis of gastric cardia adenocarcinoma.
CyclinD1 and p57(kip2) as biomarkers in differentiation, metastasis and prognosis of gastric cardia adenocarcinoma.
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DOI：
10.18632/oncotarget.18008
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发表时间：
2017-09-26
期刊：
影响因子：
--
通讯作者：
Feng XS
中科院分区：
文献类型：
--
作者：
Ru Y;Chen XJ;Zhao ZW;Zhang PF;Feng SH;Gao Q;Gao SG;Feng XS
本研究旨在探讨P57Kip2和Cyclin D1在贲门腺癌中的表达及意义。P57Kip2是细胞周期中的负调控因子。相反，Cyclin D1是细胞周期进程的正向调节因子。本研究收集了32例GCA组织及癌旁组织。采用免疫组织化学和荧光定性聚合酶链式反应方法检测GCA及癌旁组织中P57Kip2和CyclinD1的表达水平。并分析其与GCA临床病理参数的相关性，以及P57Kip2和CyclinD1在GCA中的表达关系。P57Kip2在胃癌组织中的表达明显降低(P=0.036)，且在不同分化程度之间有显著相关性(P<0.05)。此外，p57Kip2高表达患者的中位生存期为41个月。与p57Kip2mRNA低表达组(37个月，X2=4.788，P=0.029)比较，差异有统计学意义(P=0.002)，与临床分期显著相关(P<0.05)。高表达组的中位生存期为34个月，明显短于低表达组(41个月，X2=4.071，P=0.044)。P57Kip2蛋白表达与Cylind1蛋白表达无相关性(P=0.55)。P57Kip2和CyclinD1的表达可能抑制或促进GCA的发生和发展。
This study aims to investigate the expression and significance of p57kip2 and cyclinD1 in gastric cardia adenocarcinoma (GCA). p57kip2 is a negative regulator in the cell cycle. On the contrary, cyclinD1 is a positive regulator of cell cycle progression. Thirty-two cases of GCA tissues and adjacent non-cancerous tissues were collected for this study. Immunohistochemistry and fluorescence qualitative PCR was used to determine the level of p57kip2 and cyclinD1 in GCA and its adjacent non-cancerous tissues. Furthermore, the correlation between the mRNA/protein and GCA clinical pathologic parameters were analyzed, and the relationship of p57kip2 and cyclinD1 in GCA were also evaluated. The expression of p57kip2 significantly lower in GCA (P = 0.036), and there was a significant correlation in the different degrees of differentiation (P < 0.05). Furthermore, median survival time was 41 months for patients with high mRNA expression of p57kip2. This was longer compared to patients with low mRNA expression of P57kip2 (37 months, X2 = 4.788, P = 0.029).The expression of cyclinD1 was significantly higher in GCA(P = 0.002), and was significant correlated to clinical stage(P<0.05). Median survival time was 34 months in patients with high mRNA expression of cyclinD1, which was shorter than in patients with low expression of cyclinD1 mRNA (41 months, X2 = 4.071, P = 0.044). The protein expression of p57kip2 was not correlated to the protein expression of cyclinD1 (P = 0.55). The expression of p57kip2 and cyclinD1 are likely to suppress or promote the tumorigenesis and progression of GCA.
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影响因子：
6.7
作者：
Bazot Q;Paschos K;Skalska L;Kalchschmidt JS;Parker GA;Allday MJ
通讯作者：
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DOI：
10.1093/ijnp/pyu121
发表时间：
2015-01-07
期刊：
The international journal of neuropsychopharmacology
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