A widespread methylotroph acyl-homoserine lactone synthase produces a new quorum sensing signal that regulates swarming in Methylobacterium fujisawaense.
A widespread methylotroph acyl-homoserine lactone synthase produces a new quorum sensing signal that regulates swarming in Methylobacterium fujisawaense.
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DOI：
10.1128/mbio.01999-23
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发表时间：
2024-01-16
期刊：
影响因子：
6.4
通讯作者：
Puri, Aaron W.
中科院分区：
文献类型：
--
作者：
Wallace, Mike;Cummings, Dale A., Jr.;Roberts, Andrew G.;Puri, Aaron W.
甲基红菌属和甲基杆菌属的粉红色兼性甲基营养菌是在地球上许多不同环境中定殖的代谢多功能细菌。尽管它们广泛存在，但这些生物体如何相互作用及其环境的分子细节仍然研究不足。我们分析了编码N-酰基高丝氨酸内酯（酰基-HSL）的基因，在这些细菌的基因组中公布的群体感应信号酶，并确定该产品的最大家族的信号酶尚未被确定。我们确定3R-OH-5 Z-C12：1-HSL作为一种新的信号产生的这些酶使用反稳定同位素标记和结构表征质谱，核磁共振光谱，和化学合成。我们表明，在甲基营养型藤泽甲基杆菌DSM 5686，这个信号激活其同源LuxR家族转录因子，并产生一个正反馈回路。我们还发现，在这种菌株中，群体感应负调节群集运动激活一个小的蛋白质，结合预测的转录因子的表达。这些结果表征了以前未描述的，但广泛的群体感应系统所使用的粉红色兼性甲基营养菌，这有助于我们了解这些重要的细菌的化学生态。被称为粉红色兼性甲基营养菌的细菌在地球上的许多不同环境中定居，在碳循环中发挥重要作用，并在某些情况下促进植物生长。然而，人们对这些生物如何相互作用以及它们的环境知之甚少。在这项工作中，我们确定了这些细菌常用的化学信号之一，并发现这个信号控制群集运动的粉红色兼性甲基营养菌藤泽甲基杆菌DSM 5686。这项工作提供了关于这些重要细菌之间相互作用的新分子细节，并将帮助科学家预测这些相互作用以及它们从基因组测序信息中调节的群体行为。
Pink-pigmented facultative methylotrophs of the genera Methylorubrum and Methylobacterium are metabolically versatile bacteria that colonize many diverse environments on earth. Despite their widespread occurrence, the molecular details of how these organisms interact with each other and their environment remain understudied. We analyzed genes encoding N-acylhomoserine lactone (acyl-HSL) quorum sensing signal synthases in the published genomes of these bacteria and determined that the product of the largest family of signal synthases had not been characterized. We identified 3R-OH-5Z-C12:1-HSL as a novel signal produced by these synthases using inverse stable isotopic labeling and structural characterization by mass spectrometry, nuclear magnetic resonance spectroscopy, and chemical synthesis. We show that in the methylotroph Methylobacterium fujisawaense DSM5686, this signal activates its cognate LuxR-family transcription factor and is produced in a positive feedback loop. We also discover that in this strain, quorum sensing negatively regulates swarming motility by activating the expression of a small protein that binds a predicted transcription factor. These results characterize a previously undescribed, yet widespread quorum sensing system used by pink-pigmented facultative methylotrophs, which helps us understand the chemical ecology of these important bacteria. Bacteria known as pink-pigmented facultative methylotrophs colonize many diverse environments on earth, play an important role in the carbon cycle, and in some cases promote plant growth. However, little is known about how these organisms interact with each other and their environment. In this work, we identify one of the chemical signals commonly used by these bacteria and discover that this signal controls swarming motility in the pink-pigmented facultative methylotroph Methylobacterium fujisawaense DSM5686. This work provides new molecular details about interactions between these important bacteria and will help scientists predict these interactions and the group behaviors they regulate from genomic sequencing information.
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