Overexpression of a NF-YC Gene Results in Enhanced Drought and Salt Tolerance in Transgenic Seashore Paspalum
Overexpression of a NF-YC Gene Results in Enhanced Drought and Salt Tolerance in Transgenic Seashore Paspalum
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NF-YC 基因的过度表达导致转基因海滨雀稗的干旱和盐耐受性增强
DOI：
10.3389/fpls.2018.01355
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发表时间：
2018-09
影响因子：
5.6
通讯作者：
Zhenfei Guo
中科院分区：
文献类型：
--
作者：
Xueli Wu;Haifan Shi;Zhenfei Guo
海滨雀稗（Paspalum vaginatum O. Swartz）是重要的暖季型草坪草品种。在这项研究中，我们产生了过度表达 CdtNF-YC1 的转基因海滨雀稗，CdtNF-YC1 是一种来自杂交狗牙根（Cynodon dactylon × Cynodon transvaalensis）的核因子 Y 转录因子。 DNA印迹杂交和qRT-PCR分析表明CdtNF-YC1已整合到转基因海滨雀稗植物的基因组中并表达。在干旱胁迫后，野生型（WT）和转基因植物均观察到相对含水量（RWC）和存活率降低，离子渗漏增加，而转基因植物比野生型植物具有更高水平的RWC和存活率以及更低的离子渗漏。盐胁迫后，光系统II的最大光化学效率（Fv/Fm）、叶绿素浓度和存活率均下降，但转基因植物中仍保持较高水平。此外，盐处理后观察到叶和根中Na+含量增加，K+减少或不变，而转基因植物中Na+水平保持较低，K+水平以及K+/Na+比值保持较高水平。结果表明，过表达CdtNF-YC1导致转基因植物的干旱和盐耐受性增强。胁迫响应基因（包括 PvLEA3、PvP5CS1、PvABI2 和 PvDREB1B）的转录水平在干旱和盐胁迫下被诱导，并且在转基因海滨雀稗中观察到的转录水平高于野生型。结果表明，转基因海滨雀稗增强的耐旱性和耐盐性与 CdtNF-YC1 过表达导致的一系列胁迫响应基因的诱导有关。
Seashore paspalum (Paspalum vaginatum O. Swartz) is an important warm-season turfgrass species. In this study we generated transgenic seashore paspalum overexpressing CdtNF-YC1, a nuclear factor Y transcription factor from hybrid bermudagrass (Cynodon dactylon × Cynodon transvaalensis). DNA blot hybridization and qRT-PCR analysis showed that CdtNF-YC1 was integrated into the genomes of transgenic seashore paspalum plants and expressed. Reduced relative water content (RWC) and survival rate and increased ion leakage were observed in both wild type (WT) and transgenic plants after drought stress, while transgenic plants had higher levels of RWC and survival rate and lower ion leakage than the WT. Maximal photochemical efficiency of photosystem II (Fv/Fm), chlorophyll concentration and survival rate were decreased after salt stress, while higher levels were maintained in transgenic plants than in WT. In addition, an increased Na+ content and decreased or unaltered K+ in leaves and roots were observed after salt treatment, while lower level of Na+ and higher levels of K+ and K+/ Na+ ratio were maintained in transgenic plants than in WT. The results indicated that overexpressing CdtNF-YC1 resulted in enhanced drought and salt tolerance in transgenic plants. Transcript levels of stress responsive genes including PvLEA3, PvP5CS1, PvABI2, and PvDREB1B were induced in response to drought and salt stress, and higher levels were observed in transgenic seashore paspalum than in WT. The results suggest that the enhanced drought and salt tolerance in transgenic seashore paspalum is associated with induction of a series of stress responsive genes as a result of overexpression of CdtNF-YC1.
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影响因子：
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作者：
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通讯作者：
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影响因子：
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影响因子：
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作者：
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DOI：
10.1007/978-1-60761-738-9
发表时间：
2010
期刊：
--
影响因子：
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作者：
Paul J. Higgins
通讯作者：
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