Activation-tagging in indica rice identifies a novel transcription factor subunit, NF-YC13 associated with salt tolerance.
Activation-tagging in indica rice identifies a novel transcription factor subunit, NF-YC13 associated with salt tolerance.
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Indica水稻中的激活标记标识了与耐盐耐受性相关的新型转录因子亚基NF-YC13。
DOI：
10.1038/s41598-017-10022-9
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发表时间：
2017-08-24
影响因子：
4.6
通讯作者：
Kirti PB
中科院分区：
文献类型：
--
作者：
Manimaran P;Venkata Reddy S;Moin M;Raghurami Reddy M;Yugandhar P;Mohanraj SS;Balachandran SM;Kirti PB
核因子Y(NF-Y)是一种异源三聚体转录因子，具有三个不同的亚基：核因子YA、核因子YB和核因子YC。它在植物的生长发育和逆境反应中发挥着重要作用。我们早些时候已经报道了在水稻品种BPT-5204中开发功能获得突变体的情况。现在，我们从70个Ac/DS植物中筛选出927个种子进行耐盐性筛选，并鉴定出一个激活标记的耐盐性DS植株(DS-16，T3代)，该基因表达增强，属于水稻NF-Y亚家族C，被命名为OsNF-YC13。GFP-Fusion定位研究表明，该蛋白定位于细胞核和细胞质。实时表达分析证实，在盐处理过程中，OsNF-YC13的转录水平以组织特异性的方式上调。生理生化鉴定结果表明，DS-16的K+/Na+比值、脯氨酸含量、叶绿素含量、具有抗氧化活性的酶活性等指标均较对照有所提高，与耐盐性相关的基因在转录水平也有上调。对OsNF-YC13启动子区域的电子计算机分析表明，存在各种关键的胁迫响应顺式调控元件。OsNF-YC13亚基本身似乎不具有直接转录激活的能力，但似乎在反式激活过程中与B-亚基相互作用。
Nuclear factor Y (NF-Y) is a heterotrimeric transcription factor with three distinct NF-YA, NF-YB and NF-YC subunits. It plays important roles in plant growth, development and stress responses. We have reported earlier on development of gain-of-function mutants in an indica rice cultivar, BPT-5204. Now, we screened 927 seeds from 70 Ac/Ds plants for salinity tolerance and identified one activation-tagged salt tolerant DS plant (DS-16, T3 generation) that showed enhanced expression of a novel ‘histone-like transcription factor’ belonging to rice NF-Y subfamily C and was named as OsNF-YC13. Localization studies using GFP-fusion showed that the protein is localized to nucleus and cytoplasm. Real time expression analysis confirmed upregulation of transcript levels of OsNF-YC13 during salt treatment in a tissue specific manner. Biochemical and physiological characterization of the DS-16 revealed enhanced K+/Na+ ratio, proline content, chlorophyll content, enzymes with antioxidant activity etc. DS-16 also showed transcriptional up-regulation of genes that are involved in salinity tolerance. In-silico analysis of OsNF-YC13 promoter region evidenced the presence of various key stress-responsive cis-regulatory elements. OsNF-YC13 subunit alone does not appear to have the capacity for direct transcription activation, but appears to interact with the B- subunits in the process of transactivation.
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