Predictive biomarkers for the progression of ocular complications in chronic Stevens-Johnson syndrome and toxic Eeidermal necrolysis.
Predictive biomarkers for the progression of ocular complications in chronic Stevens-Johnson syndrome and toxic Eeidermal necrolysis.
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慢性史蒂文斯 - 约翰逊综合征和有毒eeidermal坏死的慢性史蒂文斯 - 约翰逊综合征中眼并发症进展的预测生物标志物。
DOI：
10.1038/s41598-020-76064-8
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发表时间：
2020-11-03
影响因子：
4.6
通讯作者：
Sotozono C
中科院分区：
文献类型：
--
作者：
Yoshikawa Y;Ueta M;Nishigaki H;Kinoshita S;Ikeda T;Sotozono C
本研究旨在通过检查泪液中的细胞因子来阐明史蒂文斯-约翰逊综合征（SJS）和中毒性表皮坏死松解症（TEN）的轻度和重度眼部并发症的预测生物标志物。在 121 只慢性期 SJS/10 只眼睛中，测量使用 Schirmer 试纸条收集的泪液样本中的细胞因子，并使用涉及 7 个组成部分（结膜化、新生血管化、浑浊化、角质化、睑缘联合和上/下结膜囊缩短）的眼表面分级评分 (OSGS) 评估眼部后遗症的严重程度，结果分为 0-3 级（最大总 OSGS： 21）。比较轻度组和重度组之间细胞因子的变化（轻度：总 OSGS 为 10 或更少，重度：总 OSGS 为 11 或更多），以及具有和不具有 7 种成分中每一种的 SJS/10 例之间的变化。在严重组中，白细胞介素（IL）-8（P < 0.01）和颗粒酶B（GrzB）（P < 0.05）显着上调。 IL-8在结膜化、新生血管或混浊眼中显着上调，GrzB在角化眼中上调，干扰素γ诱导蛋白10（IP-10）在结膜化或新生血管中下调，IL-1α在混浊眼中上调（均：P< 0.05）。 IL-8和IP-10参与结膜化和新生血管形成，而GrzB参与角质化。泪液中的 IL-8 和 GrzB 可能反映 SJS/TEN 相关眼部后遗症的严重程度。
This study aimed to clarify predictive biomarkers of mild and severe ocular complications of Stevens-Johnson syndrome (SJS) and toxic epidermal necrolysis (TEN) by examining the cytokines in tears. In 121 chronic-phase SJS/TEN eyes, cytokines in tear samples collected using Schirmer test strips were measured, and ocular sequelae severity was evaluated using an Ocular Surface Grading Score (OSGS) involving 7 components (conjunctivalization, neovascularization, opacification, keratinization, symblepharon, and upper/lower conjunctival-sac shortening), with findings categorized into grades 0–3 (maximum total OSGS: 21). Changes in cytokines between the mild and severe groups (mild: total OSGS of 10 or less, severe: total OSGS of 11 or more), and changes between SJS/TEN cases with and without each of the 7 components, were compared. In the severe group, there was significant upregulation of interleukin (IL)-8 (P < 0.01) and Granzyme B (GrzB) (P < 0.05). IL-8 was significantly upregulated in eyes with conjunctivalization, neovascularization, or opacification, GrzB was upregulated in eyes with keratinization, interferon-γ-inducible protein 10 (IP-10) was downregulated in eyes with conjunctivalization or neovascularization, and IL-1α was upregulated in eyes with opacification (all: P < 0.05). IL-8 and IP-10 was involved in conjunctivalization and neovascularization, while GrzB was involved in keratinization. IL-8 and GrzB in tears may reflect SJS/TEN-related ocular sequelae severity.
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影响因子：
4.2
作者：
Sotozono, Chie;Ueta, Mayumi;Hashimoto, Koji
通讯作者：
Hashimoto, Koji
影响因子：
4.2
作者：
Basu, Sayan;Shanbhag, Swapna S.;Sangwan, Virender S.
通讯作者：
Sangwan, Virender S.
影响因子：
3.4
作者：
Sotozono C;Inatomi T;Nakamura T;Koizumi N;Yokoi N;Ueta M;Matsuyama K;Kaneda H;Fukushima M;Kinoshita S
通讯作者：
Kinoshita S
影响因子：
13.7
作者：
Sotozono, Chie;Ang, Leonard P. K.;Kinoshita, Shigeru
通讯作者：
Kinoshita, Shigeru
影响因子：
1.6
作者：
Lehman, SS
通讯作者：
Lehman, SS