Microbicidal effect of 405-nm blue LED light on Candida albicans and Streptococcus mutans dual-species biofilms on denture base resin
Microbicidal effect of 405-nm blue LED light on Candida albicans and Streptococcus mutans dual-species biofilms on denture base resin
复制标题
405 nm 蓝光 LED 光对义齿基托树脂上白色念珠菌和变形链球菌双种生物膜的杀菌效果
DOI：
10.1007/s10103-021-03323-z
复制
发表时间：
2021
影响因子：
2.1
通讯作者：
Satomura Kazuhito
中科院分区：
文献类型：
--
作者：
Tsutsumi-Arai Chiaki;Arai Yuki;Terada-Ito Chika;Imamura Takahiro;Tatehara Seiko;Ide Shinji;Wakabayashi Noriyuki;Satomura Kazuhito
本研究研究：(1)405 nm蓝光LED光照射对双种条件下白色念珠菌和变形链球菌在义齿基托树脂上形成的生物膜的杀微生物作用；(2)照射诱导细胞内活性氧（ROS）的产生；(3)细胞内具有光敏剂作用的卟啉的存在。制备义齿基托树脂标本；albicansandS。允许在标本上形成跨双种生物膜。用405nm蓝光LED光照射生物膜，并使用菌落形成单位测定、荧光显微镜和扫描电镜（SEM）进行分析。c的单物种生物膜。albicansandS。用405nm蓝光LED光照射样品上的突变体。照射后，细胞内ROS水平升高。测量突变细胞。此外，胞内卟啉含量也呈白色差异。mutanswere测量。照射30 min以上，可明显抑制c的集落形成能力。albicansandS。变形链球菌。荧光显微镜显示几乎所有的c。albicansandS。诱变细胞被照射杀死。扫描电镜图像显示不同的细胞损伤模式。照射导致细胞内ROS的产生，两种c中均存在卟啉。albicansandS。mutanscells。综上所述，405 nm蓝光发光二极管光照射40 min可有效消毒tc。albicanshyphae and。改变双物种生物膜，并可能与细胞内卟啉反应，导致每个微生物产生ROS。
This study investigated: (1) the microbicidal effect of 405-nm blue LED light irradiation on biofilm formed byCandida albicanshyphae andStreptococcus mutansunder dual-species condition on denture base resin, (2) the generation of intracellular reactive oxygen species (ROS) induced by irradiation, and (3) the existence of intracellular porphyrins, which act as a photosensitizer. Denture base resin specimens were prepared andC. albicansandS. mutansdual-species biofilms were allowed to form on the specimens. The biofilms were irradiated with 405-nm blue LED light and analyzed using the colony-forming unit assay, fluorescence microscopy, and scanning electron microscopy (SEM). Single-species biofilms ofC. albicansandS. mutansformed on the specimens were irradiated with 405-nm blue LED light. After the irradiation, the intracellular ROS levels inC. albicansandS. mutanscells were measured. In addition, the level of intracellular porphyrins inC. albicansandS. mutanswere measured. Irradiation for more than 30 min significantly inhibited the colony formation ability ofC. albicansandS. mutans. Fluorescence microscopy revealed that almost allC. albicansandS. mutanscells were killed by irradiation. SEM images showed various cell damage patterns. Irradiation led to the generation of intracellular ROS and porphyrins were present in bothC. albicansandS. mutanscells. In conclusion, irradiation with 405-nm blue light-emitting diode light for 40 min effectively disinfectC. albicanshyphae andS. mutansdual-species biofilms and possibly react with intracellular porphyrins resulting in generation of ROS in each microorganism.
登录
查看更多内容
义齿性口炎患者念珠菌血清学研究。
DOI：
--
发表时间：
1982
期刊：
Scandinavian journal of dental research
影响因子：
--
作者：
T. Bergendal;K. Holmberg
通讯作者：
K. Holmberg
影响因子：
2.2
作者：
Hoenes, K.;Hess, M.;Hessling, M.
通讯作者：
Hessling, M.
DOI：
10.3889/oamjms.2016.089
发表时间：
2016-09-15
影响因子：
--
作者：
Moussa AR;Dehis WM;Elboraey AN;ElGabry HS
通讯作者：
ElGabry HS
用于有机硅上混合多物种口咽生物膜组合物的生长培养基
影响因子：
--
作者：
M. Leonhard;Beata Zatorska;D. Moser;B. Schneider
通讯作者：
B. Schneider
影响因子：
3.1
作者：
Falsetta, Megan L.;Klein, Marlise I.;Koo, Hyun
通讯作者：
Koo, Hyun