Pectin methylesterase and pectin remodelling differ in the fibre walls of two gossypium species with very different fibre properties.
Pectin methylesterase and pectin remodelling differ in the fibre walls of two gossypium species with very different fibre properties.
复制标题
DOI：
10.1371/journal.pone.0065131
复制
发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Llewellyn DJ
中科院分区：
文献类型：
--
作者：
Liu Q;Talbot M;Llewellyn DJ
果胶是双子叶植物初生细胞壁的主要成分，在高尔基体中合成，以甲酯形式分泌到细胞壁中，随后通过果胶甲酯酶（PME）脱酯化。已知PME对果胶的重塑在调节植物细胞扩增中是重要的，但在棉花中的研究很少。在这项研究中，全基因组分析表明，PMEs是一个大的多基因家族（81个基因）在二倍体棉花（Gossypium raimondii），扩大了66在拟南芥中，并提出了新的功能在棉花的进化。基于EST丰度，相对较少的PME基因在纤维中高度表达，并且从两个棉花物种中克隆并测序了纤维中最丰富的五个PME基因。它们的显着的序列差异和它们在一个物种内的纤维中的阶段特异性表达表明纤维发育过程中的亚专业化。我们确定了转录丰度的五个纤维PME，总PME酶活性，果胶含量和程度的果胶在纤维壁的两个棉花品种在第一个25-30天的纤维生长。纤维PME的转录丰度和总PME酶活性在G. barbadense（Gb）比G.在纤维伸长的后期，尤其是在纤维伸长的后期。在Gh和Gb的纤维伸长过程中（5-12 dpa），总果胶高，但脱酯化果胶低。去酯化果胶水平上升，此后当总PME活性增加，这发生在Gb纤维导致在17和22 dpa之间的Gb纤维酯化程度较低。Gb纤维比Gh纤维更细更长，因此在向壁增厚过渡期间果胶重塑的差异可能是影响最终纤维直径和长度（棉纤维的两个关键质量属性）的重要因素。
Pectin, a major component of the primary cell walls of dicot plants, is synthesized in Golgi, secreted into the wall as methylesters and subsequently de-esterified by pectin methylesterase (PME). Pectin remodelling by PMEs is known to be important in regulating cell expansion in plants, but has been poorly studied in cotton. In this study, genome-wide analysis showed that PMEs are a large multi-gene family (81 genes) in diploid cotton (Gossypium raimondii), an expansion over the 66 in Arabidopsis and suggests the evolution of new functions in cotton. Relatively few PME genes are expressed highly in fibres based on EST abundance and the five most abundant in fibres were cloned and sequenced from two cotton species. Their significant sequence differences and their stage-specific expression in fibres within a species suggest sub-specialisation during fibre development. We determined the transcript abundance of the five fibre PMEs, total PME enzyme activity, pectin content and extent of de-methylesterification of the pectin in fibre walls of the two cotton species over the first 25–30 days of fibre growth. There was a higher transcript abundance of fibre-PMEs and a higher total PME enzyme activity in G. barbadense (Gb) than in G. hirsutum (Gh) fibres, particularly during late fibre elongation. Total pectin was high, but de-esterified pectin was low during fibre elongation (5–12 dpa) in both Gh and Gb. De-esterified pectin levels rose thereafter when total PME activity increased and this occurred earlier in Gb fibres resulting in a lower degree of esterification in Gb fibres between 17 and 22 dpa. Gb fibres are finer and longer than those of Gh, so differences in pectin remodelling during the transition to wall thickening may be an important factor in influencing final fibre diameter and length, two key quality attributes of cotton fibres.
登录
查看更多内容
影响因子：
4.8
作者：
Catoire, L;Pierron, M;Goldberg, R
通讯作者：
Goldberg, R
影响因子：
3.5
作者：
Johansson, K;El-Ahmad, M;Eklund, H
通讯作者：
Eklund, H
影响因子：
5.6
作者：
Jenkins, J;Mayans, O;Pickersgill, RW
通讯作者：
Pickersgill, RW
影响因子：
3.7
作者：
Avci U;Pattathil S;Singh B;Brown VL;Hahn MG;Haigler CH
通讯作者：
Haigler CH
影响因子：
11.6
作者：
Di Matteo, A;Giovane, A;Tsernoglou, D
通讯作者：
Tsernoglou, D