Cotton fiber cell walls of Gossypium hirsutum and Gossypium barbadense have differences related to loosely-bound xyloglucan.
Cotton fiber cell walls of Gossypium hirsutum and Gossypium barbadense have differences related to loosely-bound xyloglucan.
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DOI：
10.1371/journal.pone.0056315
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Haigler CH
中科院分区：
文献类型：
--
作者：
Avci U;Pattathil S;Singh B;Brown VL;Hahn MG;Haigler CH
棉纤维具有特殊的长度和厚度，是一种重要的天然纺织纤维。这些特性主要是通过初级和次级细胞壁的合成而产生的。商业棉纤维是一层纤维次生壁，由一层薄的角质化的初生壁包围，但关于任何棉花品种的纤维细胞壁中的多糖都只有很少的细节。此外，众所周知，陆地棉(Gh)纤维具有一种粘性棉纤维中层(CFML)，它将相邻的纤维连接成组织样束，但尚不清楚其他重要的商业棉纤维中是否存在CFML。我们比较了Gh和Gossypium barbadense(GB)这两个最重要的商业棉种在高度控制的温室中平行生长时纤维发育过程中的细胞壁化学。在这些生长条件下，两种纤维的早期伸长率和次生壁沉积的开始时间相似，但不出所料，GB纤维的伸长期比Gh纤维延长，品质更高。GB纤维有CFML，但它不是纤维伸长所直接需要的，因为GB纤维在CFML水解后继续快速伸长。对于这两个物种，用四种日益强烈的溶剂提取了七个年龄段的纤维，然后使用基于抗体的糖凝胶图谱分析了细胞壁基质多糖表位。结合纤维切片的免疫组织化学染色，结果表明，与Gh纤维相比，GB纤维的CFML中木糖葡聚糖含量较低。GB纤维的木聚糖内水解酶活性也较高。总体而言，这些数据为这两个最重要的商业棉花品种的细胞壁结构提供了丰富的相似和不同之处。
Cotton fiber is an important natural textile fiber due to its exceptional length and thickness. These properties arise largely through primary and secondary cell wall synthesis. The cotton fiber of commerce is a cellulosic secondary wall surrounded by a thin cuticulated primary wall, but there were only sparse details available about the polysaccharides in the fiber cell wall of any cotton species. In addition, Gossypium hirsutum (Gh) fiber was known to have an adhesive cotton fiber middle lamella (CFML) that joins adjacent fibers into tissue-like bundles, but it was unknown whether a CFML existed in other commercially important cotton fibers. We compared the cell wall chemistry over the time course of fiber development in Gh and Gossypium barbadense (Gb), the two most important commercial cotton species, when plants were grown in parallel in a highly controlled greenhouse. Under these growing conditions, the rate of early fiber elongation and the time of onset of secondary wall deposition were similar in fibers of the two species, but as expected the Gb fiber had a prolonged elongation period and developed higher quality compared to Gh fiber. The Gb fibers had a CFML, but it was not directly required for fiber elongation because Gb fiber continued to elongate rapidly after CFML hydrolysis. For both species, fiber at seven ages was extracted with four increasingly strong solvents, followed by analysis of cell wall matrix polysaccharide epitopes using antibody-based Glycome Profiling. Together with immunohistochemistry of fiber cross-sections, the data show that the CFML of Gb fiber contained lower levels of xyloglucan compared to Gh fiber. Xyloglucan endo-hydrolase activity was also higher in Gb fiber. In general, the data provide a rich picture of the similarities and differences in the cell wall structure of the two most important commercial cotton species.
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影响因子：
4.4
作者：
Alabady, Magdy S.;Youn, Eunseog;Wilkins, Thea A.
通讯作者：
Wilkins, Thea A.
影响因子：
2.3
作者：
Hequet, Eric F.;Wyatt, Bobby;Thibodeaux, Devron P.
通讯作者：
Thibodeaux, Devron P.
影响因子：
5.6
作者：
Haigler CH;Betancur L;Stiff MR;Tuttle JR
通讯作者：
Tuttle JR
影响因子：
32.5
作者：
DeMartini, Jaclyn D.;Pattathil, Sivakumar;Wyman, Charles E.
通讯作者：
Wyman, Charles E.
DOI：
10.1002/9781444391015.ch1
发表时间：
2011-01-01
期刊：
PLANT POLYSACCHARIDES, BIOSYNTHESIS AND BIOENGINEERING
影响因子：
--
作者：
Fry, Stephen C.
通讯作者：
Fry, Stephen C.