Simian-Human immunodeficiency viruses expressing chimeric subtype B/C Vpu proteins demonstrate the importance of the amino terminal and transmembrane domains in the rate of CD4(+) T cell loss in macaques.
Simian-Human immunodeficiency viruses expressing chimeric subtype B/C Vpu proteins demonstrate the importance of the amino terminal and transmembrane domains in the rate of CD4(+) T cell loss in macaques.
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DOI：
10.1016/j.virol.2012.10.030
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发表时间：
2013-01-20
期刊：
影响因子：
3.7
通讯作者：
Stephens EB
中科院分区：
文献类型：
--
作者：
Ruiz A;Schmitt K;Culley N;Stephens EB
Previously, we reported that simian-human immunodeficiency viruses expressing either the lab adapted subtype B (SHIVKU-1bMC33) or subtype C (SHIVSCVpu) Vpu proteins of human immunodeficiency virus type 1 (HIV-1) had different rates of CD4+ T cell loss following inoculation into macaques. In this study, we have generated SHIVs that express either the subtype B or subtype C N-terminal (NTD) and transmembrane (TMD) domains and the opposing cytoplasmic domain (SHIVVpuBC, SHIVVpuCB). In culture systems, the SHIVVpuBC replicated faster than SHIVVpuCB while both proteins exhibited similar ability to down-modulate CD4 surface expression. Following inoculation into macaques, SHIVVpuBC resulted in rapid CD4+ T cell loss similar to the parental SHIVKU-1bMC33 while the rate of CD4+ T cell loss in those inoculated with SHIVVpuCB was intermediate of SHIVSCVpu and SHIVKU-1bMC33. These results emphasize the importance of the Vpu NTD/TMD region in the rate of CD4+ T cell loss in the pathogenic X4 SHIV/macaque model.
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影响因子：
3.7
作者：
Hill, M. Sarah;Ruiz, Autumn;Stephens, Edward B.
通讯作者：
Stephens, Edward B.
影响因子：
6.4
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John-Stewart, GC;Nduati, RW;Overbaugh, J
通讯作者：
Overbaugh, J
影响因子：
5.4
作者：
Ariën, KK;Abraha, A;Arts, EJ
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Arts, EJ
影响因子：
6.7
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McNatt, Matthew W.;Zang, Trinity;Hatziioannou, Theodora;Bartlett, Mackenzie;Ben Fofana, Ismael;Johnson, Welkin E.;Neil, Stuart J. D.;Bieniasz, Paul D.
通讯作者：
Bieniasz, Paul D.
影响因子：
6.7
作者：
Gupta RK;Hué S;Schaller T;Verschoor E;Pillay D;Towers GJ
通讯作者：
Towers GJ