Macromolecular connections of active zone material to docked synaptic vesicles and presynaptic membrane at neuromuscular junctions of mouse.
Macromolecular connections of active zone material to docked synaptic vesicles and presynaptic membrane at neuromuscular junctions of mouse.
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DOI：
10.1002/cne.21975
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发表时间：
2009-04-10
期刊：
影响因子：
--
通讯作者：
McMahan UJ
中科院分区：
文献类型：
--
作者：
Nagwaney S;Harlow ML;Jung JH;Szule JA;Ress D;Xu J;Marshall RM;McMahan UJ
用电子断层扫描观察小鼠神经肌肉接头轴突终末中组成活动区物质(AZM)的大分子。在突触传递过程中，大分子之间的相互连接、与突触前膜上的钙通道以及与膜上停靠的突触小泡的连接，与先前用电子断层扫描观察到的青蛙神经肌肉接头处的AZM大分子的连接相似，支持AZM调节囊泡对接和融合的假说。相对于停靠的小泡，Azm排列的物种差异可能有助于解释停靠时更大的囊泡-突触前膜接触面积，以及在突触传递期间更大的融合可能性。小鼠体内的某些AZM大分子连接到突触小泡，在不发生融合的部位接触突触前膜。这些二次对接的小泡与膜和Azm大分子的关系与一次对接的小泡不同，这与它们具有不同的Azm调节行为相一致。
Electron tomography was used to view macromolecules composing active zone material (AZM) in axon terminals at mouse neuromuscular junctions. Connections of the macromolecules to each other, to calcium channels in the presynaptic membrane and to synaptic vesicles docked on the membrane prior to fusing with it during synaptic transmission were similar to those of AZM macromolecules at frog neuromuscular junctions previously examined by electron tomography and support the hypothesis that AZM regulates vesicle docking and fusion. A species difference in the arrangement of AZM relative to docked vesicles may help account for a greater vesicle-presynaptic membrane contact area during docking and a greater probability of fusion during synaptic transmission in mouse. Certain AZM macromolecules in mouse were connected to synaptic vesicles contacting the presynaptic membrane at sites where fusion does not occur. These secondary docked vesicles had a different relationship to the membrane and AZM macromolecules than primary docked vesicles consistent with their having a different AZM-regulated behavior.
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