Brassinosteroids can regulate cellulose biosynthesis by controlling the expression of CESA genes in Arabidopsis.
Brassinosteroids can regulate cellulose biosynthesis by controlling the expression of CESA genes in Arabidopsis.
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油菜素类固醇可以通过控制拟南芥中CESA基因的表达来调节纤维素生物合成
DOI：
10.1093/jxb/err164
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发表时间：
2011-08
影响因子：
6.9
通讯作者：
Wang X
中科院分区：
文献类型：
--
作者：
Xie L;Yang C;Wang X
植物激素油菜素类固醇(BRs)在调节细胞伸长和细胞大小方面起着重要作用，BR相关突变体在拟南芥中表现出明显的矮化表型。纤维素是一种生物聚合物，在细胞扩张和伸长过程中对细胞壁的形成有重要贡献。然而，BRS是否调节纤维素的合成，如果是的话，BRS诱导细胞伸长的潜在机制尚不清楚。测定了BR相关突变体和野生型突变体的纤维素含量和纤维素合成酶基因(CESA)的表达水平。染色质免疫沉淀(ChIP)实验和遗传分析证实了BRs对CESA基因的调控。在此发现，BR缺陷型或BR感受型突变株的纤维素含量低于野生型。DET2-1和BRI1-301中CESA基因，尤其是与初级细胞壁合成相关的CESA基因的表达降低，并且仅在BR缺失突变体Det2-1中被BRs诱导。芯片实验表明，BR激活的转录因子BES1可以与大多数CESA基因的上游元件结合，特别是与初级细胞壁相关的元件。此外，BR受体BRI1在CESA1、3和6突变体中的过度表达只能部分挽救矮生表型。我们的发现为BRs调节纤维素合成以完成植物发育中的细胞伸长过程的机制提供了潜在的见解。
The phytohormones, brassinosteroids (BRs), play important roles in regulating cell elongation and cell size, and BR-related mutants in Arabidopsis display significant dwarf phenotypes. Cellulose is a biopolymer which has a major contribution to cell wall formation during cell expansion and elongation. However, whether BRs regulate cellulose synthesis, and if so, what the underlying mechanism of cell elongation induced by BRs is, is unknown. The content of cellulose and the expression levels of the cellulose synthase genes (CESAs) was measured in BR-related mutants and their wild-type counterpart. The chromatin immunoprecipitation (CHIP) experiments and genetic analysis were used to demonstrate that BRs regulate CESA genes. It was found here that the BR-deficient or BR-perceptional mutants contain less cellulose than the wild type. The expression of CESA genes, especially those related to primary cell wall synthesis, was reduced in det2-1 and bri1-301, and was only inducible by BRs in the BR-deficient mutant det2-1. CHIP experiments show that the BR-activated transcription factor BES1 can associate with upstream elements of most CESA genes particularly those related with the primary cell wall. Furthermore, over-expression of the BR receptor BRI1 in CESA1, 3, and 6 mutants can only partially rescue the dwarf phenotypes. Our findings provide potential insights into the mechanism that BRs regulate cellulose synthesis to accomplish the cell elongation process in plant development.
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影响因子：
64.5
作者：
Nemhauser, Jennifer L.;Hong, Fangxin;Chory, Joanne
通讯作者：
Chory, Joanne
影响因子：
56.9
作者：
He, JX;Gendron, JM;Wang, ZY
通讯作者：
Wang, ZY
DOI：
10.1073/pnas.0706569104
发表时间：
2007-09-25
影响因子：
11.1
作者：
Desprez, Thierry;Juraniec, Michal;Vernhettes, Samantha
通讯作者：
Vernhettes, Samantha
影响因子：
11.6
作者：
Mitsuda, N;Seki, M;Ohme-Takagi, M
通讯作者：
Ohme-Takagi, M
影响因子：
2.9
作者：
Laurentin, A;Edwards, CA
通讯作者：
Edwards, CA