TYPLEX® Chelate, a novel feed additive, inhibits Campylobacter jejuni biofilm formation and cecal colonization in broiler chickens.
TYPLEX® Chelate, a novel feed additive, inhibits Campylobacter jejuni biofilm formation and cecal colonization in broiler chickens.
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DOI：
10.3382/ps/pex413
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发表时间：
2018-04-01
期刊：
影响因子：
4.4
通讯作者：
Mahdavi J
中科院分区：
文献类型：
--
作者：
Khattak F;Paschalis V;Green M;Houdijk JGM;Soultanas P;Mahdavi J
减少弯曲杆菌属。家禽运输具有挑战性，但对于控制全球人类细菌性胃肠炎的这一主要原因至关重要。尽管人们对弯曲杆菌属的机制和途径了解很多。由于在家禽中定植，关于对抗弯曲杆菌属的无抗生素解决方案的文献很少。在家禽中定植。我们进行了体外和体内研究，以研究新型饲料添加剂 TYPLEX® 螯合物（酪氨酸铁）在抑制空肠弯曲杆菌 (C. jejuni) 生物膜形成以及减少上市日龄肉鸡中空肠弯曲杆菌 (C. jejuni) 和大肠杆菌 (E. coli) 定植方面的作用。在一项体外研究中，使用塑料珠测定法研究了对空肠弯曲菌生物膜形成的抑制作用。结果表明 TYPLEX® 螯合物显着减少生物膜的形成。在一项体内研究中，800 只肉鸡（一岁）被随机分配到采用随机区组设计的 4 种饮食治疗中，每个治疗有 10 个重复栏，每栏 20 只鸡。在第 21 天，所有鸡都通过带种子的垫料接受空肠弯曲菌的攻击。第 42 天，收集盲肠样本并测试挥发性脂肪酸 (VFA) 浓度以及空肠弯曲菌和大肠杆菌计数。结果表明，TYPLEX® 螯合剂可将家禽中每克盲肠样本的空肠弯曲菌和大肠杆菌携带量分别减少 2 和 1 log10，并提高盲肠 VFA 浓度。这些发现支持 TYPLEX® 螯合物作为一种新型非抗生素饲料添加剂，可能有助于生产具有较低弯曲杆菌病公共卫生风险的家禽。
Reducing Campylobacter spp. carriage in poultry is challenging, but essential to control this major cause of human bacterial gastroenteritis worldwide. Although much is known about the mechanisms and route of Campylobacter spp. colonization in poultry, the literature is scarce on antibiotic-free solutions to combat Campylobacter spp. colonization in poultry. In vitro and in vivo studies were conducted to investigate the role of TYPLEX® Chelate (ferric tyrosine), a novel feed additive, in inhibiting Campylobacter jejuni (C. jejuni) biofilm formation and reducing C. jejuni and Escherichia coli (E. coli) colonization in broiler chickens at market age. In an in vitro study, the inhibitory effect on C. jejuni biofilm formation using a plastic bead assay was investigated. The results demonstrated that TYPLEX® Chelate significantly reduces biofilm formation. In an in vivo study, 800 broilers (one d old) were randomly allocated to 4 dietary treatments in a randomized block design, each having 10 replicate pens with 20 birds per pen. At d 21, all birds were challenged with C. jejuni via seeded litter. At d 42, cecal samples were collected and tested for volatile fatty acid (VFA) concentrations and C. jejuni and E. coli counts. The results showed that TYPLEX® Chelate reduced the carriage of C. jejuni and E. coli in poultry by 2 and 1 log10 per gram cecal sample, respectively, and increased cecal VFA concentrations. These findings support TYPLEX® Chelate as a novel non-antibiotic feed additive that may help produce poultry with a lower public health risk of Campylobacteriosis.
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影响因子：
3.3
作者：
Calderon-Gomez, Lirio I.;Hartley, Lauren E.;Korolik, Victoria
通讯作者：
Korolik, Victoria
影响因子：
3.3
作者：
Andreoletti, Olivier;Budka, Herbert;Vanopdenbosch, Emmanuel
通讯作者：
Vanopdenbosch, Emmanuel
影响因子：
3.2
作者：
Kalmokoff, Martin;Lanthier, Patricia;Szymanski, Christine M.
通讯作者：
Szymanski, Christine M.
DOI：
10.1016/j.ijfoodmicro.2014.07.024
发表时间：
2014-10-01
影响因子：
5.4
作者：
Duffy, Lesley L.;Blackall, Patrick J.;Fegan, Narelle
通讯作者：
Fegan, Narelle
影响因子：
2
作者：
Allain, V.;Chemaly, M.;Le Bouquin, S.
通讯作者：
Le Bouquin, S.