Trophectoderm mechanics direct epiblast shape upon embryo implantation.
Trophectoderm mechanics direct epiblast shape upon embryo implantation.
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DOI：
10.1016/j.celrep.2020.108655
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发表时间：
2021-01-19
期刊：
影响因子：
8.8
通讯作者：
Zernicka-Goetz M
中科院分区：
文献类型：
--
作者：
Weberling A;Zernicka-Goetz M
着床是哺乳动物胚胎发生的一个标志，在此期间胚胎与母体子宫内膜建立联系，重塑并进行生长和分化。胚胎在这一转变过程中改变形状的机制和事件序列在很大程度上尚不清楚。在这里，我们表明，第一个胚外世系，极地滋养外胚层，是重塑胚胎上皮细胞的关键调节因素。在免疫手术或抑制剂治疗后，其功能的丧失阻止了上胚层形状的转变。在小鼠中，极地滋养外胚层对上胚层施加物理力量，使其从椭圆形转变为杯状。在人类胚胎中，极地滋养外胚层的行为与之相反，施加了一种拉伸力。通过在小鼠胚胎发生中模拟这种拉伸行为，我们可以指导外胚细胞在这个阶段采用人类胚胎的盘状形状特征。因此，极地滋养外胚层对上胚层形状起着保守的调节作用。小鼠上皮细胞从胚泡到卵柱的重建经历了5个阶段，植入时的重建不是胚胎谱系所固有的，极地滋养外胚层介导了表皮细胞形状的获得，极地滋养外胚层对表皮细胞形状的调节在进化中似乎是保守的，Weberling和Zericka-Goetz表征了小鼠着床后上皮细胞的重建。其从椭圆形到杯状的转变是由极地滋养外胚层组织驱动的。与人类胚胎和抑制物处理的比较分析表明，极地滋养外胚层在植入时是一种进化上保守的上胚层形状调节器。
Implantation is a hallmark of mammalian embryogenesis during which embryos establish their contacts with the maternal endometrium, remodel, and undertake growth and differentiation. The mechanisms and sequence of events through which embryos change their shape during this transition are largely unexplored. Here, we show that the first extraembryonic lineage, the polar trophectoderm, is the key regulator for remodeling the embryonic epiblast. Loss of its function after immuno-surgery or inhibitor treatments prevents the epiblast shape transitions. In the mouse, the polar trophectoderm exerts physical force upon the epiblast, causing it to transform from an oval into a cup shape. In human embryos, the polar trophectoderm behaves in the opposite manner, exerting a stretching force. By mimicking this stretching behavior in mouse embryogenesis, we could direct the epiblast to adopt the disc-like shape characteristic of human embryos at this stage. Thus, the polar trophectoderm acts as a conserved regulator of epiblast shape. Mouse epiblast remodeling from blastocyst to egg cylinder is achieved in five stages Epiblast remodeling upon implantation is not inherent to the embryonic lineage The polar trophectoderm mediates epiblast shape acquisition Epiblast shape regulation by the polar trophectoderm appears conserved in evolution Weberling and Zernicka-Goetz characterize the remodeling of the mouse epiblast upon implantation. Its transformation from oval to cup shaped is driven by the polar trophectoderm tissue. Comparative analyses with human embryos and inhibitor treatments suggest the polar trophectoderm as an evolutionarily conserved regulator of epiblast shape upon implantation.
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DOI：
10.1083/jcb.201001149
发表时间：
2010-06-28
期刊：
The Journal of cell biology
影响因子：
--
作者：
le Duc Q;Shi Q;Blonk I;Sonnenberg A;Wang N;Leckband D;de Rooij J
通讯作者：
de Rooij J
DOI：
10.1073/pnas.1700934114
发表时间：
2017-06-27
影响因子：
11.1
作者：
Galea, Gabriel L.;Cho, Young-June;Copp, Andrew J.
通讯作者：
Copp, Andrew J.
影响因子：
23.9
作者：
Nichols, Jennifer;Smith, Austin
通讯作者：
Smith, Austin
影响因子：
3.9
作者：
Nelson, W. James
通讯作者：
Nelson, W. James
影响因子：
10.5
作者：
FASSLER, R;MEYER, M
通讯作者：
MEYER, M