Vinculin potentiates E-cadherin mechanosensing and is recruited to actin-anchored sites within adherens junctions in a myosin II-dependent manner.
Vinculin potentiates E-cadherin mechanosensing and is recruited to actin-anchored sites within adherens junctions in a myosin II-dependent manner.
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DOI：
10.1083/jcb.201001149
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发表时间：
2010-06-28
期刊：
影响因子：
--
通讯作者：
de Rooij J
中科院分区：
文献类型：
--
作者：
le Duc Q;Shi Q;Blonk I;Sonnenberg A;Wang N;Leckband D;de Rooij J
E-cadherin定位于承受张力的细胞-细胞连接处，以帮助响应机械应力将信号从E-钙粘蛋白传递到肌动蛋白细胞骨架。细胞表面受体整合化学和机械信号以调节广泛的生物过程。整合素复合物是细胞外基质和肌动球蛋白细胞骨架之间的机械转换器。通过类比，钙粘蛋白复合物可能在细胞-细胞连接处起机械传感器的作用，但钙粘蛋白的这种能力尚未得到直接证明。此外，维持这种功能所需的E-钙粘蛋白和肌动蛋白之间连接的分子组成尚未解决。在这项研究中，我们描述了纳米力学测量表明，E-钙粘蛋白复合物是功能mechanosensors，F-肌动蛋白和E-钙粘蛋白之间传递力。成像实验表明，细胞间力与黏着斑蛋白在肌动蛋白锚定的钙粘蛋白粘附处的积累相一致，纳米力学测量表明黏着斑蛋白增强了E-钙粘蛋白的机械感觉反应。这些研究直接证明了E-钙粘蛋白复合物的机械感觉能力，并确定了黏着斑蛋白在细胞-细胞连接处的新功能。这些发现对屏障功能、形态发生、细胞迁移和侵袭有影响，并可能扩展到所有经典钙粘蛋白调节细胞-细胞粘附的软组织。
Vinculin localizes to tension-bearing cell–cell junctions to help transmit signals from E-cadherin to the actin cytoskeleton in response to mechanical stress. Cell surface receptors integrate chemical and mechanical cues to regulate a wide range of biological processes. Integrin complexes are the mechanotransducers between the extracellular matrix and the actomyosin cytoskeleton. By analogy, cadherin complexes may function as mechanosensors at cell–cell junctions, but this capacity of cadherins has not been directly demonstrated. Furthermore, the molecular composition of the link between E-cadherin and actin, which is needed to sustain such a function, is unresolved. In this study, we describe nanomechanical measurements demonstrating that E-cadherin complexes are functional mechanosensors that transmit force between F-actin and E-cadherin. Imaging experiments reveal that intercellular forces coincide with vinculin accumulation at actin-anchored cadherin adhesions, and nanomechanical measurements show that vinculin potentiates the E-cadherin mechanosensory response. These investigations directly demonstrate the mechanosensory capacity of the E-cadherin complex and identify a novel function for vinculin at cell–cell junctions. These findings have implications for barrier function, morphogenesis, cell migration, and invasion and may extend to all soft tissues in which classical cadherins regulate cell–cell adhesion.
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影响因子：
64.5
作者：
Choquet, D;Felsenfeld, DP;Sheetz, MP
通讯作者：
Sheetz, MP
影响因子：
64.5
作者：
Drees, F;Pokutta, S;Weis, WI
通讯作者：
Weis, WI
影响因子：
3.7
作者：
Miyake, Yuka;Inoue, Naoko;Yonemura, Shigenobu
通讯作者：
Yonemura, Shigenobu
钙粘蛋白介导的细胞分选不能由结合或粘附特异性确定。
影响因子：
7.8
作者：
Niessen, Carien M;Gumbiner, Barry M
通讯作者：
Gumbiner, Barry M
在哺乳动物上皮细胞中钙粘着蛋白细胞接触的形态发生过程中，肌球蛋白VI和VINCULIN合作。
DOI：
10.1083/jcb.200612042
发表时间：
2007-07-30
期刊：
The Journal of cell biology
影响因子：
--
作者：
Maddugoda MP;Crampton MS;Shewan AM;Yap AS
通讯作者：
Yap AS