Fabrication and mechanical evaluation of anatomically-inspired quasilaminate hydrogel structures with layer-specific formulations.
Fabrication and mechanical evaluation of anatomically-inspired quasilaminate hydrogel structures with layer-specific formulations.
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DOI：
10.1007/s10439-012-0666-5
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发表时间：
2013-02
影响因子：
3.8
通讯作者：
Grande-Allen, K. Jane
中科院分区：
文献类型：
--
作者：
Tseng, Hubert;Cuchiara, Maude L.;Durst, Christopher A.;Cuchiara, Michael P.;Lin, Chris J.;West, Jennifer L.;Grande-Allen, K. Jane
组织工程的一个主要挑战是创造具有代表天然组织（如心脏瓣膜小叶、血管和软骨）的层特异性机械性能的多层支架。为此，聚乙二醇二丙烯酸酯（PEGDA）水凝胶由于其可调节的机械和生物特性是有吸引力的材料。本研究探讨了三层水凝胶准层合材料的制备。设计了一种新型的夹心法来制备具有不同刚度层的准层合板。三层结构由两个“硬”外层和一个“软”内层组成。双层准层合材料的拉伸测试表明，这些支架不会在界面处失效。弯曲试验表明，脱细胞准层合材料的弯曲模量介于“硬”和“软”水凝胶层的弯曲模量之间。相同配方的三层凝胶与相同配方的单层凝胶相比，其弯曲模量和膨胀率无显著差异。细胞的包封和水凝胶层内酚红的加入降低了三层支架的弯曲模量。所提供的数据表明，这种制造方法可以制造出具有坚固界面的准层合材料，将不同力学性能和生物功能的层结合在一起，从而为更准确地代表天然组织的多层层合支架奠定基础。
A major tissue engineering challenge is the creation of multilaminate scaffolds with layer-specific mechanical properties representative of native tissues, such as heart valve leaflets, blood vessels, and cartilage. For this purpose, poly(ethylene glycol) diacrylate (PEGDA) hydrogels are attractive materials due to their tunable mechanical and biological properties. This study explored the fabrication of trilayer hydrogel quasilaminates. A novel sandwich method was devised to create quasilaminates with layers of varying stiffnesses. The trilayer structure was comprised of two “stiff” outer layers and one “soft” inner layer. Tensile testing of bilayer quasilaminates demonstrated that these scaffolds do not fail at the interface. Flexural testing showed that the bending modulus of acellular quasilaminates fell between the bending moduli of the “stiff” and “soft” hydrogel layers. The bending modulus and swelling of trilayer scaffolds with the same formulations were not significantly different than single layer gels of the same formulation. The encapsulation of cells and the addition of phenol red within the hydrogel layers decreased bending modulus of the trilayer scaffolds. The data presented demonstrates that this fabrication method can make quasilaminates with robust interfaces, integrating layers of different mechanical properties and biofunctionalization, and thus forming the foundation for a multilaminate scaffold that more accurately represents native tissue.
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影响因子：
14
作者：
Cuchiara, Michael P.;Allen, Alicia C. B.;West, Jennifer L.
通讯作者：
West, Jennifer L.
影响因子：
3.4
作者：
Hoffmann, Joseph C.;West, Jennifer L.
通讯作者：
West, Jennifer L.
影响因子：
7
作者：
Chou AI;Akintoye SO;Nicoll SB
通讯作者：
Nicoll SB
影响因子：
14
作者：
Mirnajafi, A;Raymer, J;Sacks, MS
通讯作者：
Sacks, MS
影响因子：
4.2
作者：
Gloeckner, DC;Billiar, KL;Sacks, MS
通讯作者：
Sacks, MS