Global Analysis of Transcriptome and Translatome Revealed That Coordinated WNT and FGF Regulate the Carapacial Ridge Development of Chinese Soft-Shell Turtle.
Global Analysis of Transcriptome and Translatome Revealed That Coordinated WNT and FGF Regulate the Carapacial Ridge Development of Chinese Soft-Shell Turtle.
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DOI：
10.3390/ijms222212441
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发表时间：
2021-11-18
影响因子：
5.6
通讯作者：
Gui JF
中科院分区：
文献类型：
--
作者：
Zhang J;Yu P;Zhao Y;Zhou Q;Yang J;Hu Q;Liu T;Bao C;Su S;Gui JF
海龟的甲壳由严重变形的融合的背椎骨、肋骨和骨板组成。特别是，在背侧躯干的龟肋骨浅层的横向生长导致肩胛骨和骨盆的包裹。最近的研究表明，甲脊（CR）是一种新的上皮-间充质转化模型，对于肋骨的排列至关重要。因此，有必要探讨龟背嵴发育的调控机制，分析龟背的形成过程。然而，目前的理解的调控网络的龟背甲脊发展是穷人由于缺乏系统的基因筛选在不同的背甲脊发展阶段和基因功能验证研究。本研究采用RNA测序和核糖体新生链复合物mRNA测序技术，对不同发育阶段的中华鳖背甲脊组织进行全基因组基因转录和翻译谱分析。转录组和翻译组的相关性分析显示，有129，670和135共差异表达基因，包括同向和反向差异表达基因，在三个对照组中，分别。对来自京都基因和基因组百科全书的共差异表达基因的途径富集分析表明，参与背甲嵴发育的信号途径发生了动态变化。特别是Wnt信号通路和MAPK信号通路可能在龟背嵴发育中起重要作用。此外，Wnt和Fgf在背甲嵴发育过程中均有表达。此外，我们发现Wnt 5a通过Wnt 5a/JNK途径调控背甲嵴的发育。因此，我们的研究揭示了鳖背甲的形态发生可能是通过保守的WNT和FGF信号通路之间的协同作用而起作用的。本研究揭示了影响中华鳖背甲嵴发育的动态信号通路，为揭示中华鳖背甲形态发生的分子机制提供了新的思路。
The turtle carapace is composed of severely deformed fused dorsal vertebrae, ribs, and bone plates. In particular, the lateral growth in the superficial layer of turtle ribs in the dorsal trunk causes an encapsulation of the scapula and pelvis. The recent study suggested that the carapacial ridge (CR) is a new model of epithelial–mesenchymal transition which is essential for the arrangement of the ribs. Therefore, it is necessary to explore the regulatory mechanism of carapacial ridge development to analyze the formation of the turtle shell. However, the current understanding of the regulatory network underlying turtle carapacial ridge development is poor due to the lack of both systematic gene screening at different carapacial ridge development stages and gene function verification studies. In this study, we obtained genome-wide gene transcription and gene translation profiles using RNA sequencing and ribosome nascent-chain complex mRNA sequencing from carapacial ridge tissues of Chinese soft-shell turtle at different development stages. A correlation analysis of the transcriptome and translatome revealed that there were 129, 670, and 135 codifferentially expressed genes, including homodirection and opposite-direction differentially expressed genes, among three comparison groups, respectively. The pathway enrichment analysis of codifferentially expressed genes from the Kyoto Encyclopedia of Genes and Genomes showed dynamic changes in signaling pathways involved in carapacial ridge development. Especially, the results revealed that the Wnt signaling pathway and MAPK signaling pathway may play important roles in turtle carapacial ridge development. In addition, Wnt and Fgf were expressed during the carapacial ridge development. Furthermore, we discovered that Wnt5a regulated carapacial ridge development through the Wnt5a/JNK pathway. Therefore, our studies uncover that the morphogenesis of the turtle carapace might function through the co-operation between conserved WNT and FGF signaling pathways. Consequently, our findings revealed the dynamic signaling pathways acting on the carapacial ridge development of Chinese soft-shell turtle and provided new insights into uncover the molecular mechanism underlying turtle shell morphogenesis.
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影响因子：
64.8
作者：
Glinka, A;Wu, W;Niehrs, C
通讯作者：
Niehrs, C
DOI：
10.1073/pnas.1200421109
发表时间：
2012-03-13
影响因子：
11.1
作者：
Ho, Hsin-Yi Henry;Susman, Michael W.;Greenberg, Michael Eldon
通讯作者：
Greenberg, Michael Eldon
影响因子：
4.6
作者：
Gao, Bo;Ajima, Rieko;Yang, Yingzi
通讯作者：
Yang, Yingzi
影响因子：
12.3
作者：
Kim D;Pertea G;Trapnell C;Pimentel H;Kelley R;Salzberg SL
通讯作者：
Salzberg SL
影响因子：
4.4
作者：
Cao, Xin;Guo, Zhong;Zhang, Gong
通讯作者：
Zhang, Gong