Interactive relationship between basement-membrane development and sarcomerogenesis in single cardiomyocytes.
Interactive relationship between basement-membrane development and sarcomerogenesis in single cardiomyocytes.
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DOI：
10.1016/j.yexcr.2014.08.020
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发表时间：
2015-01-01
影响因子：
3.7
通讯作者：
Gao BZ
中科院分区：
文献类型：
--
作者：
Yang H;Borg TK;Liu H;Gao BZ
心脏基底膜（BM）是位于肌膜外侧的细胞外基质（ECM）的高度组织层，主要由层粘连蛋白和IV型胶原组成，它们组装成致密、组织良好的网络包围每个成年心肌细胞的表面。心脏 BM 的发育通过肌节和整合素之间的相互作用在心肌器官发生中发挥关键作用。由于心肌纤维结构复杂且缺乏适当的研究方法，BM 发育、肌节生长和整合素表达之间的详细相互作用仍不清楚。在这项研究中，新鲜分离的 3 天新生儿心肌细胞 (CM) 在对齐的胶原蛋白上培养，模拟体内 ECM 结构并诱导新生儿 CM 生长成杆状形状。然后使用共聚焦显微镜对培养4-72小时的新生儿CM上的层粘连蛋白和α-肌动蛋白或整合素β1进行双荧光免疫染色成像，并通过共定位分析评估层粘连蛋白沉积和α-肌动蛋白表达之间的空间关系。 4小时后，层粘连蛋白沉积在Z体（点状α-肌动蛋白）和整合素周围；从18小时到72小时，其与Z线（线状α-肌动蛋白）和整合素的逐渐共定位增加了皮尔逊系数；这表明从新生儿阶段到成年期的BM网络的发育与通过整合素介导的相互作用的肌节形成密切相关。
The cardiac basement membrane (BM), the highly organized layer of the extracellular matrix (ECM) on the external side of the sarcolemma, is mainly composed of laminin and collagen IV, which assemble a dense, well-organized network to surround the surface of each adult cardiomyocyte. The development of the cardiac BM plays a key role in organogenesis of the myocardium through interactions between sarcomeres and integrins. Because of the complicated structure of cardiac muscle fibers and lack of a proper investigation method, the detailed interactions among BM development, sarcomeric growth, and integrin expression remain unclear. In this study, freshly isolated 3-day neonatal cardiomyocytes (CMs) were cultured on aligned collagen, which mimics the in vivo ECM structure and induces neonatal CMs to grow into rod-like shapes. Then double fluorescence-immunostained laminin and α-actinin or integrin β1 on neonatal CMs cultured 4-72 h were imaged using a confocal microscope, and the spatial relationship between laminin deposition and α-actinin expression was evaluated by colocalization analysis. At 4 h, laminin was deposited around Z-bodies (dot-shaped α-actinin) and integrins; from 18-to-72 h, its gradual colocalization with Z-lines (line-shaped α-actinin) and integrins increased Pearson's coefficient; this indicates that development of the BM network from the neonatal stage to adulthood is closely related to sarcomeric formation via integrin-mediated interactions.
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影响因子：
20.1
作者：
Israeli-Rosenberg S;Manso AM;Okada H;Ross RS
通讯作者：
Ross RS
影响因子：
3.7
作者：
Miner, Jeffrey H.
通讯作者：
Miner, Jeffrey H.
层粘连蛋白聚合诱导受体细胞骨架网络。
影响因子：
7.8
作者：
Colognato, H;Winkelmann, D A;Yurchenco, P D
通讯作者：
Yurchenco, P D
影响因子：
3.4
作者：
Rodriguez, Anthony G.;Han, Sangyoon J.;Sniadecki, Nathan J.
通讯作者：
Sniadecki, Nathan J.
影响因子：
56.9
作者：
LANGER, GA;FRANK, JS;SERAYDARIAN, K
通讯作者：
SERAYDARIAN, K