The use of selective adsorbents in capillary electrophoresis‐mass spectrometry for analyte preconcentration and microreactions: A powerful three‐dimensional tool for multiple chemical and biological applications
The use of selective adsorbents in capillary electrophoresis‐mass spectrometry for analyte preconcentration and microreactions: A powerful three‐dimensional tool for multiple chemical and biological applications
复制标题
在毛细管电泳-质谱分析中使用选择性吸附剂进行分析物预浓缩和微反应：用于多种化学和生物应用的强大三维工具
作者：
N. Guzman;R. Stubbs
最近，许多注意力集中在使用基于化学或生物特异性的选择性吸附剂的在线样品预浓缩和毛细管电泳中微反应的开发和应用。基本原理涉及两个相互作用的化学或生物系统，彼此之间具有高选择性和亲和力。自然界中的这些分子相互作用通常涉及非共价和可逆的化学过程。正确结合到固体支持物上，“亲和配体”可以选择性地吸附简单或复杂混合物中各种浓度的“目标分析物”。结果，分离的分析物得到富集和高度纯化。当这种允许非共价化学相互作用和生化反应发生的亲和技术与高分辨率毛细管电泳和质谱在线耦合时，就创建了化学和生物信息的强大工具。本文描述了在线高分辨率分离的生物识别和亲和相互作用的概念、“分析物浓缩器-微反应器”的制造、吸附和解吸的优化条件、与质谱的耦合以及临床和制药兴趣的各种应用。
Much attention has recently been directed to the development and application of on‐line sample preconcentration and microreactions in capillary electrophoresis using selective adsorbents based on chemical or biological specificity. The basic principle involves two interacting chemical or biological systems with high selectivity and affinity for each other. These molecular interactions in nature usually involve noncovalent and reversible chemical processes. Properly bound to a solid support, an “affinity ligand” can selectively adsorb a “target analyte” found in a simple or complex mixture at a wide range of concentrations. As a result, the isolated analyte is enriched and highly purified. When this affinity technique, allowing noncovalent chemical interactions and biochemical reactions to occur, is coupled on‐line to high‐resolution capillary electrophoresis and mass spectrometry, a powerful tool of chemical and biological information is created. This paper describes the concept of biological recognition and affinity interaction on‐line with high‐resolution separation, the fabrication of an “analyte concentrator‐microreactor”, optimization conditions of adsorption and desorption, the coupling to mass spectrometry, and various applications of clinical and pharmaceutical interest.
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作者：
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DOI：
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期刊：
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影响因子：
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期刊：
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