A new set of primers for COI amplification from purpleback flying squid (Sthenoteuthis oualaniensis).
A new set of primers for COI amplification from purpleback flying squid (Sthenoteuthis oualaniensis).
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一套新的引物,用于紫背乌贼 (Sthenoteuthis oualaniensis) 的 COI 扩增
DOI:
10.1080/23802359.2017.1357439
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发表时间:
2017-07-25
期刊:
影响因子:
--
通讯作者:
Du F
中科院分区:
文献类型:
--
作者:
Xu L;Huang Q;Xu S;Wang X;Zhang P;Xu L;Du F
Abstract Despite the contribution of DNA barcoding towards understanding the biodiversity and distribution of species, the success of the mitochondrial cytochrome c oxidase subunit I gene (COI) amplification has been quite variable when it comes to Cephalopoda. Some species in this class such as Sthenoteuthis oualaniensis seem to be more difficult to amplify COI than others due to failed amplifications with universal primer and lack of specific set of primers. In this study, we developed new Sthenoteuthis – specific primer set, which significantly increased average amplification success. The new primer set will aid the recovery of barcodes from this difficult group and facilitate further studies in phylogeny and cryptic diversity of Sthenoteuthis oualaniensis.
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DOI:
10.1098/rsbl.2003.0025
发表时间:
2003-08-07
影响因子:
4.7
作者:
Hebert, PDN;Ratnasingham, S;deWaard, JR
通讯作者:
deWaard, JR
DOI:
10.1111/j.1471-8286.2007.01678.x
发表时间:
2007-05-01
期刊:
Molecular ecology notes
影响因子:
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作者:
Ratnasingham S;Hebert PD
通讯作者:
Hebert PD
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1998-12-07
影响因子:
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