A new set of primers for COI amplification from purpleback flying squid (Sthenoteuthis oualaniensis).

A new set of primers for COI amplification from purpleback flying squid (Sthenoteuthis oualaniensis).
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一套新的引物,用于紫背乌贼 (Sthenoteuthis oualaniensis) 的 COI 扩增

DOI:
10.1080/23802359.2017.1357439
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发表时间:
2017-07-25
期刊:
Mitochondrial DNA. Part B, Resources
影响因子:
--
通讯作者:
Du F
Du F
中科院分区:
其他
文献类型:
--
作者:
Xu L;Huang Q;Xu S;Wang X;Zhang P;Xu L;Du F

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摘要尽管DNA条形码有助于了解物种的生物多样性和分布,但在鲸足类中,线粒体细胞色素c氧化酶亚基I基因(COI)扩增的成功与否一直存在很大差异。由于通用引物扩增失败和缺乏特异性引物,这类中的一些物种如Sthenoteuthis oualaniensis似乎比其他物种更难扩增COI。在这项研究中,我们开发了新的Sthenoteuthis特异性引物组,显着提高平均扩增成功率。新的引物组将有助于从这个困难的群体中恢复条形码,并促进进一步研究Sthenoteuthis oualaniensis的遗传和隐藏多样性。
Abstract Despite the contribution of DNA barcoding towards understanding the biodiversity and distribution of species, the success of the mitochondrial cytochrome c oxidase subunit I gene (COI) amplification has been quite variable when it comes to Cephalopoda. Some species in this class such as Sthenoteuthis oualaniensis seem to be more difficult to amplify COI than others due to failed amplifications with universal primer and lack of specific set of primers. In this study, we developed new Sthenoteuthis – specific primer set, which significantly increased average amplification success. The new primer set will aid the recovery of barcodes from this difficult group and facilitate further studies in phylogeny and cryptic diversity of Sthenoteuthis oualaniensis.
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