The hepatic lectin of zebrafish binds a wide range of bacteria and participates in immune defense
The hepatic lectin of zebrafish binds a wide range of bacteria and participates in immune defense
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斑马鱼的肝凝集素与多种细菌结合并参与免疫防御
DOI：
10.1016/j.fsi.2018.08.012
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发表时间：
2018-11
影响因子：
4.7
通讯作者：
Li Hongyan
中科院分区：
文献类型：
--
作者：
Yang Qingyun;Wang Peng;Wang Su;Wang Yashuo;Feng Shuoqi;Zhang Shicui;Li Hongyan
C型凝集素（C-type lectin，CTL）具有多种功能，包括细胞间粘附、免疫应答和细胞凋亡。去唾液酸糖蛋白受体（ASGPR），又称肝凝集素，是第一个被发现的动物凝集素，但在硬骨鱼类中的研究还很有限。在这项研究中，我们确定了一个假定的蛋白质，命名为斑马鱼肝凝集素（Zhl）。Thezhl编码一种典型的钙依赖性碳水化合物结合蛋白，主要在肝脏中以组织特异性方式表达。LPS和LTA攻击导致zh表达显著上调，表明zh参与免疫应答。事实上，Zhl的重组C型凝集素结构域（rCTLD）被发现能够凝集和结合革兰氏阴性和革兰氏阳性细菌，并增强巨噬细胞对细菌的吞噬作用。此外，rCTLD特异性结合不溶性脂多糖（LPS），脂磷壁酸（LTA）和肽聚糖（PGN），这是由半乳糖抑制。有趣的是，Zhl位于膜上，并且其过表达可以抑制炎症前细胞因子的产生。综上所述，这些结果表明Zhl具有能够防御入侵病原体的免疫活性，丰富了我们对ASGPR/肝凝集素功能的理解。
C-type lectins (CTLs) have a diverse range of functions including cell-cell adhesion, immune response to pathogens and apoptosis. Asialoglycoprotein receptor (ASGPR), also known as hepatic lectin, a member of CTLs, was the first animal lectin identified, yet information regarding it remains rather limited in teleost. In this study, we identified a putative protein in zebrafish, named as the zebrafish hepatic lectin (Zhl). Thezhlencoded a typical Ca2+-dependent carbohydrate-binding protein, and was mainly expressed in the liver in a tissue specific fashion. Challenge with LPS and LTA resulted in significant up-regulation ofzhlexpression, suggesting involvement in immune response. Actually, recombinant C-type lectin domain (rCTLD) of Zhl was found to be capable of agglutinating and binding to both Gram-negative and Gram-positive bacteria and enhancing the phagocytosis of the bacteria by macrophages. Moreover, rCTLD specifically bound to insoluble lipopolysaccharide (LPS), lipoteichoic acid (LTA) and peptidoglycan (PGN), which were inhibited by galactose. Interestingly, Zhl was located in the membrane, and its overexpression could inhibit the production of pre-inflammatory cytokines. Taken together, these results indicate that Zhl has immune activity capable of defending invading pathogens, enriching our understanding of the function of ASGPR/hepatic lectin.
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影响因子：
2.5
作者：
Ghazarian, Haike;Idoni, Brian;Oppenheimer, Steven B.
通讯作者：
Oppenheimer, Steven B.
RIG-I 样受体的免疫信号传导。
影响因子：
32.4
作者：
Loo YM;Gale M Jr
通讯作者：
Gale M Jr
纤连蛋白和去唾液酸蛋白受体介导乙型肝炎表面抗原与细胞表面的结合
DOI：
10.1007/s00705-010-0657-5
发表时间：
2010-04
期刊：
Arch Virol
影响因子：
--
作者：
杨静
通讯作者：
杨静
影响因子：
2.9
作者：
Shu C;Wang S;Xu T
通讯作者：
Xu T
影响因子：
3.8
作者：
BECKER, S;SPIESS, M;KLENK, HD
通讯作者：
KLENK, HD