Targeted molecular trait stacking in cotton through targeted double-strand break induction.
Targeted molecular trait stacking in cotton through targeted double-strand break induction.
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DOI：
10.1111/pbi.12085
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发表时间：
2013-10
影响因子：
13.8
通讯作者：
Broadhvest J
中科院分区：
文献类型：
--
作者：
D'Halluin K;Vanderstraeten C;Van Hulle J;Rosolowska J;Van Den Brande I;Pennewaert A;D'Hont K;Bossut M;Jantz D;Ruiter R;Broadhvest J
最近靶向基因组修改工具的发展导致了如何将多个性状结合起来的新概念。靶向基因组修饰是基于使用具有量身定制的特异性的核酸酶在特定的目标位点引入DNA双链断裂(DSB)。设计了一种重新设计的巨核酸酶，用于特异切割棉花中转基因昆虫控制基因位点附近的内源靶序列。靶向DNA切割和同源重组介导的修复相结合，使得额外性状基因(HPPD、epsps)的精确靶向插入在棉花中成为可能。在独立转化的胚性愈伤组织系中，以大约2%的频率恢复了定向插入事件。我们进一步证明，所有性状基因都是作为单个遗传单位遗传的，这将简化未来的多性状导入。
Recent developments of tools for targeted genome modification have led to new concepts in how multiple traits can be combined. Targeted genome modification is based on the use of nucleases with tailor-made specificities to introduce a DNA double-strand break (DSB) at specific target loci. A re-engineered meganuclease was designed for specific cleavage of an endogenous target sequence adjacent to a transgenic insect control locus in cotton. The combination of targeted DNA cleavage and homologous recombination–mediated repair made precise targeted insertion of additional trait genes (hppd, epsps) feasible in cotton. Targeted insertion events were recovered at a frequency of about 2% of the independently transformed embryogenic callus lines. We further demonstrated that all trait genes were inherited as a single genetic unit, which will simplify future multiple-trait introgression.
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影响因子：
14.9
作者：
Epinat, JC;Arnould, S;Lacroix, E
通讯作者：
Lacroix, E
影响因子：
5.1
作者：
Cai, Charles Q.;Doyon, Yannick;Petolino, Joseph F.
通讯作者：
Petolino, Joseph F.
影响因子：
56.9
作者：
Jinek, Martin;Chylinski, Krzysztof;Charpentier, Emmanuelle
通讯作者：
Charpentier, Emmanuelle
影响因子：
3.7
作者：
GAMBORG, OL;MILLER, RA;OJIMA, K
通讯作者：
OJIMA, K
影响因子：
10.5
作者：
Day, CD;Lee, E;Ow, DW
通讯作者：
Ow, DW