Vasa nucleates asymmetric translation along the mitotic spindle during unequal cell divisions.
Vasa nucleates asymmetric translation along the mitotic spindle during unequal cell divisions.
复制标题
在不等细胞分裂期间，Vasa使有丝分裂纺锤体沿着不对称平移成核。
DOI：
10.1038/s41467-022-29855-8
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发表时间：
2022-04-20
影响因子：
16.6
通讯作者：
中科院分区：
文献类型：
--
作者：
纺锤体上的mRNA翻译被假设为是细胞调节剂的本地化生产的必要策略。这种机制可能是重要的，特别是在早期胚胎细胞，其中有一个大的扩散体积，并进行快速的细胞分裂。然而，检验这一假设的证据有限。在这里，我们使用一个胚胎与对称和不对称的细胞分裂和操纵瓦萨蛋白，RNA解旋酶，在活海胆胚胎的纺锤体。我们了解到，纺锤体是翻译的主要位点，单个纺锤体内的蛋白质合成可能是不平等的，有助于在胚胎发生过程中驱动不对称的细胞分裂。将Vasa募集到异位亚细胞区域诱导了一个新的翻译位点，扰乱了纺锤体上的不对称翻译，并改变了细胞的命运。基于这些观察结果，我们得出结论，Vasa的功能在本地化的翻译，这提供了一个时空控制蛋白质合成，是至关重要的快速发育的胚胎细胞。mRNA翻译与有丝分裂纺锤体的关联被认为参与细胞命运决定因子的局部产生。在这里，作者表明Vasa促进了不对称翻译，这有助于海胆胚胎发生过程中的差异调节。
mRNA translation on the spindle is hypothesized to be an essential strategy for the localized production of cell regulators. This mechanism may be important particularly in early embryonic cells, which have a large diffusion volume and that undergo rapid cell divisions. Evidence to test such a hypothesis has been, however, limited. Here, we use an embryo with both symmetric and asymmetric cell divisions and manipulate Vasa protein, an RNA-helicase, on the spindle in live sea urchin embryos. We learned that the spindle serves as a major site of translation and that protein synthesis within a single spindle can be unequal and help drive asymmetric cell divisions during embryogenesis. Recruiting Vasa to the ectopic sub-cellular region induced a new site of translation, disturbed asymmetric translation on the spindle, and changed the cell fate. Based on these observations, we conclude that Vasa functions in localized translation, which provides a spatiotemporal control in protein synthesis and is essential for rapidly developing embryonic cells. Association of mRNA translation with the mitotic spindle is thought to be involved in localized production of cell fate determinants. Here, the authors show Vasa facilitates asymmetric translation, which contributes to differential regulation during sea urchin embryogenesis.
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影响因子：
48
作者：
Kennedy, Matthew J.;Hughes, Robert M.;Peteya, Leslie A.;Schwartz, Joel W.;Ehlers, Michael D.;Tucker, Chandra L.
通讯作者：
Tucker, Chandra L.
影响因子：
64.5
作者：
HAY, B;JAN, LY;JAN, YN
通讯作者：
JAN, YN
DOI：
10.1083/jcb.201302139
发表时间：
2013-09-30
期刊：
The Journal of cell biology
影响因子：
--
作者：
Kotani T;Yasuda K;Ota R;Yamashita M
通讯作者：
Yamashita M
影响因子：
16
作者：
Mizuno, H;Mal, TK;Miyawakil, A
通讯作者：
Miyawakil, A
影响因子：
4.6
作者：
Oulhen, Nathalie;Swartz, S. Zachary;Wessel, Gary M.
通讯作者：
Wessel, Gary M.