A novel CC chemokine ligand 2 like gene from ayu Plecoglossus altivelis is involved in the innate immune response against to Vibrio anguillarum
A novel CC chemokine ligand 2 like gene from ayu Plecoglossus altivelis is involved in the innate immune response against to Vibrio anguillarum
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来自香鱼的新型CC趋化因子配体2样基因参与针对鳗弧菌的先天免疫反应
DOI：
10.1016/j.fsi.2019.02.019
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发表时间：
2019-04
影响因子：
4.7
通讯作者：
Chen J
中科院分区：
文献类型：
--
作者：
Yu L;Li C H;Chen J
趋化因子（Csingle bond dc motif）配体2 (CCL2)，又称单核细胞趋化蛋白1 (MCP-1)，是调控哺乳动物单核/巨噬细胞（MO/MФ）迁移和浸润的关键趋化因子之一。然而，鱼类CCL2的功能仍不清楚。在此，我们鉴定了一种新的ccl2样蛋白（PaCCL2L）的cDNA序列。序列分析显示，PaCCL2L与CCL2同源物归为一类，与墨西哥四鱼（Astyanax mexicanus）和斑马鱼（Danio rerio）同源物亲缘关系最近。PaCCL2转录本在健康鱼的所有组织中均有表达，其中在脾脏中表达水平最高。在鳗弧菌感染后，PaCCL2L转录本在测试组织中显著增加，包括肝脏、脾脏和头肾。然后通过原核表达获得重组PaCCL2L成熟肽（rPaCCL2L），并生成相应的抗体（抗PaCCL2L）。在ayu MO/MФ中，PaCCL2L蛋白和mRNA的表达显著增加。anguillarumchallenge。腹腔注射rPaCCL2L显著提高了鳗鲡的存活率，降低了组织细菌负荷。rPaCCL2L对体外和体内MO/MΦ和中性粒细胞的趋化性均有积极作用。同时，rPaCCL2L对体外lps刺激的MO/MΦ （M1型）有正向趋化作用，而对camp刺激的MO/MΦ （M2型）无趋化作用。此外，rPaCCL2L处理对MO/MΦ吞噬、细菌杀伤、呼吸爆发和促炎细胞因子mRNA表达有增强作用，而抗paccl2l处理有抑制作用。我们的研究表明，PaCCL2L可能在ayu对v的免疫应答中发挥作用。通过趋化招募和活化MO/MΦ。
Chemokine (Csingle bondC motif) ligand 2 (CCL2), also known as monocyte chemoattractant protein 1 (MCP-1), is one of the key chemokines that regulate migration and infiltration of monocytes/macrophages (MO/MФ) in mammals. However, the functional repertoire of fish CCL2 remains unclear. Here, we identified a cDNA sequence encoding a novel CCL2-like protein (PaCCL2L) in ayu,Plecoglossus altivelis. Sequence analysis revealed that PaCCL2L grouped with CCL2 homologs, and is most closely related to Mexican tetra (Astyanax mexicanus) and zebrafish (Danio rerio) homologs. PaCCL2 transcripts were expressed in all tested tissues from healthy ayu, with the highest level in the spleen. Upon Vibrio anguillarum infection, PaCCL2L transcripts increased significantly in tested tissues, including the liver, spleen, and head kidney. We then produced the recombinant PaCCL2L mature peptide (rPaCCL2L) by prokaryotic expression and generated the corresponding antibodies (anti-PaCCL2L). A significant increase in PaCCL2L protein and mRNA expression was observed in ayu MO/MФ followingV. anguillarumchallenge. Intraperitoneal injection of rPaCCL2L resulted in significantly improved survival and reduced tissue bacterial load inV. anguillarum-infected ayu. rPaCCL2L had a positive effect on the chemotaxis of MO/MΦ and neutrophils bothin vitroandin vivo. Meanwhile, rPaCCL2L exhibited a positive effect on the chemotaxis of LPS-stimulated MO/MΦ (M1 type)in vitro, whereas it exhibited no chemotaxis effect on cAMP-stimulated MO/MΦ (M2 type). In addition, rPaCCL2L treatment exhibited an enhanced effect on MO/MΦ phagocytosis, bacterial killing, respiratory burst, and mRNA expression of proinflammatory cytokines, whereas anti-PaCCL2L treatment had an inhibitory effect. Our study demonstrates that PaCCL2L might play a role in the immune response of ayu againstV. anguillaruminfection through chemotactic recruitment and activation of MO/MΦ.
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DOI：
10.4049/jimmunol.178.9.5828
发表时间：
2007-05
期刊：
The Journal of Immunology
影响因子：
--
作者：
C. Winter;K. Taut;Mrigank Srivastava;F. Länger;M. Mack;D. Briles;J. Paton;R. Maus;T. Welte;M. Gunn;U. Maus
通讯作者：
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影响因子：
3.7
作者：
Rong YJ;Lu XJ;Chen J
通讯作者：
Chen J
影响因子：
20.3
作者：
B. Rollins;A. Walz;M. Baggiolini
通讯作者：
B. Rollins;A. Walz;M. Baggiolini
影响因子：
2.2
作者：
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通讯作者：
K. Bacon;M. Baggiolini;H. Broxmeyer;R. Horuk;I. Lindley;A. Mantovani;K. Maysushima;P. Murphy;
DOI：
10.4049/jimmunol.0804096
发表时间：
2009-04
期刊：
The Journal of Immunology
影响因子：
--
作者：
C. Winter;Wiebke Herbold;R. Maus;F. Länger;D. Briles;J. Paton;T. Welte;U. Maus
通讯作者：
C. Winter;Wiebke Herbold;R. Maus;F. Länger;D. Briles;J. Paton;T. Welte;U. Maus