Methods of Palate Culture in Later Palatogenesis: Elevation, Horizontal Outgrowth, and Fusion.

Methods of Palate Culture in Later Palatogenesis: Elevation, Horizontal Outgrowth, and Fusion.
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腭发育后期的腭培养方法:抬高、水平生长和融合。

DOI:
10.1007/978-1-0716-1847-9_6
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发表时间:
2022
期刊:
Methods in molecular biology (Clifton, N.J.)
影响因子:
--
通讯作者:
Morgan JD
Morgan JD
中科院分区:
--
文献类型:
--
作者:
Morgan JD

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腭部的体外培养为研究腭裂的发生提供了一种通用的模型。腭裂畸形仍然是全球最常见的出生缺陷之一,在正常和畸形腭裂的未来研究中有很大的空间。这一过程可以在小鼠模型中使用上颌外植体的高氧滚动培养和Trowell类型的静态培养来研究,这两种培养方法分别适用于研究上颌骨发育的不同阶段。在这里,我们描述了两种方法:前者用于研究腭架抬高和水平生长,后者用于研究腭架融合。这两种方法都适用于使用非专业设备培养胚胎13.5天的小鼠胚胎。
The ex vivo culture of the palate has provided a versatile model in which to study palatogenesis. Dysmorphias of the palate remain one of the most common birth defects globally, with great scope for future research in both normal and dysmorphic palatogenesis. This process can be studied in the mouse model using both the hyperoxic rolling culture of maxillary explants and Trowell-type static cultures, which are optimal for the study of different stages of palate development respectively. Here, we describe both methods: the former for the study of palatal shelf elevation and horizontal growth, and the latter for palatal shelf fusion . Both are applicable in murine embryos cultured at embryonic day 13.5 using nonspecialist equipment.
DOI: 10.1007/s00429-004-0379-0
发表时间: 2004-04-01
期刊: ANATOMY AND EMBRYOLOGY
影响因子: --
作者:
Chou, MJ;Kosazuma, T;Shiota, K
通讯作者: Shiota, K
DOI: 10.1242/dev.00950
发表时间: 2004
影响因子: 3.9
作者:
R. Quinlan;Paul Martin;A. Graham
通讯作者: A. Graham