AtNSF regulates leaf serration by modulating intracellular trafficking of PIN1 in Arabidopsis thaliana.
AtNSF regulates leaf serration by modulating intracellular trafficking of PIN1 in Arabidopsis thaliana.
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AtNSF 通过调节拟南芥细胞内 PIN1 运输来调节叶片锯齿
DOI：
10.1111/jipb.13043
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发表时间：
2020-12-02
影响因子：
11.4
通讯作者：
Li X
中科院分区：
文献类型：
--
作者：
Tang LP;Yang Y;Wang H;Li L;Liu L;Liu Y;Yuan J;Zhao XY;Palme K;Su YH;Li X
在真核生物中，N-乙基马来酰亚胺敏感因子（NSF）是一种保守的AAA+ ATP酶，是促进分泌囊泡与靶膜融合的膜运输机制的关键组分。在这里，我们证明了拟南芥基因组中含有一个单一的拷贝的NSF，AtNSF，它在调节叶锯齿中起着至关重要的作用。AtNSF敲除突变体atnsf-1在叶缘表现出更多的锯齿。此外，在atnsf-1突变体中，PIN-FORMED 1（PIN 1）生长素外排转运蛋白的极性定位在atnsf-1叶片的边缘周围扩散，根生长受到抑制。更多的PIN 1-GFP积累在atnsf-1植物的细胞内区室中，表明AtNSF是PIN在内体和质膜之间的细胞内运输所必需的。此外，在atnsf-1 pin 1 - 8双突变体中，锯齿表型受到抑制，这表明AtNSF是PIN 1介导的极性生长素运输调节叶片锯齿所必需的。杯形子叶2（CUC 2）转录因子基因在atnsf-1植物中上调，cuc 2 - 3单突变体表现出光滑的叶缘，表明AtNSF也在CUC 2途径中发挥作用。我们的研究结果表明，AtNSF调节PIN 1产生的生长素最大与CUC 2介导的反馈回路，以控制叶片锯齿。N-乙基马来酰亚胺敏感因子（NSF）促进真核生物中分泌囊泡的融合。在拟南芥中，AtNSF通过影响PIN-FORMED 1生长素转运蛋白的运输和干扰PIN-FORMED 1和杯形子叶2介导的叶锯齿反馈回路来负调控叶锯齿。
In eukaryotes, N‐ethylmaleimide‐sensitive factor (NSF) is a conserved AAA+ATPase and a key component of the membrane trafficking machinery that promotes the fusion of secretory vesicles with target membranes. Here, we demonstrate that the Arabidopsis thaliana genome contains a single copy of NSF, AtNSF, which plays an essential role in the regulation of leaf serration. The AtNSF knock‐down mutant, atnsf‐1, exhibited more serrations in the leaf margin. Moreover, polar localization of the PIN‐FORMED1 (PIN1) auxin efflux transporter was diffuse around the margins of atnsf‐1 leaves and root growth was inhibited in the atnsf‐1 mutant. More PIN1‐GFP accumulated in the intracellular compartments of atnsf‐1 plants, suggesting that AtNSF is required for intracellular trafficking of PIN between the endosome and plasma membrane. Furthermore, the serration phenotype was suppressed in the atnsf‐1 pin1‐8 double mutant, suggesting that AtNSF is required for PIN1‐mediated polar auxin transport to regulate leaf serration. The CUP‐SHAPED COTYLEDON2 (CUC2) transcription factor gene is up‐regulated in atnsf‐1 plants and the cuc2‐3 single mutant exhibits smooth leaf margins, demonstrating that AtNSF also functions in the CUC2 pathway. Our results reveal that AtNSF regulates the PIN1‐generated auxin maxima with a CUC2‐mediated feedback loop to control leaf serration. N‐ethylmaleimide‐sensitive factor (NSF) promotes the fusion of secretory vesicles in eukaryotes. In Arabidopsis, AtNSF negatively regulates leaf serration by affecting trafficking of the PIN‐FORMED1 auxin transporter and interfering with the PIN‐FORMED1 and CUP‐SHAPED COTYLEDON2‐mediated feedback loop for leaf serration.
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影响因子：
64.8
作者：
Blilou, I;Xu, J;Scheres, B
通讯作者：
Scheres, B
影响因子：
64.5
作者：
Geldner, N;Anders, N;Jürgens, G
通讯作者：
Jürgens, G
影响因子：
4.6
作者：
Hay, Angela;Barkoulas, Michalis;Tsiantis, Miltos
通讯作者：
Tsiantis, Miltos
影响因子：
9.9
作者：
Kleine-Vehn, Juergen;Wabnik, Krzysztof;Martiniere, Alexandre;Langowski, Lukasz;Willig, Katrin;Naramoto, Satoshi;Leitner, Johannes;Tanaka, Hirokazu;Jakobs, Stefan;Robert, Stephanie;Luschnig, Christian;Govaerts, Willy;Hell, Stefan W.;Runions, John;Friml, Jiri
通讯作者：
Friml, Jiri
DOI：
10.1073/pnas.1424856112
发表时间：
2015-02-17
影响因子：
11.1
作者：
Doyle, Siamsa M.;Haeger, Ash;Robert, Stephanie
通讯作者：
Robert, Stephanie