Suitability of EGCG as a Means of Stabilizing a Porcine Osteochondral Xenograft.
Suitability of EGCG as a Means of Stabilizing a Porcine Osteochondral Xenograft.
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DOI：
10.3390/jfb8040043
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发表时间：
2017-09-23
影响因子：
4.8
通讯作者：
Gloth P
中科院分区：
文献类型：
--
作者：
Elder S;Clune J;Walker J;Gloth P
由于非交联骨软骨异种移植物的力学性能不如天然软骨，并且容易过早降解，因此我们寻求一种安全有效的异种移植物稳定方法。本研究的目的是评估表没食子儿茶素没食子酸酯（EGCG）通过胶原交联稳定脱细胞猪骨软骨异种移植物的能力。我们的目的是评估EGCG对交联度、机械性能、胶原酶抗性、细胞毒性和体外生物相容性的影响。表没食子儿茶素没食子酸酯是一种绿色茶多酚，可作为胶原交联剂。将猪骨软骨栓脱细胞，然后通过浸泡在EGCG中交联。胶原酶暴露后的交联度、软骨压缩刚度、软骨-骨界面强度、摩擦系数和残余质量均随着表没食子酸没食子酸酯浓度的增加而增加。除了摩擦系数外，EGCG处理可以将机械性能恢复到等于或超过天然软骨的水平。表没食子儿茶素没食子酸酯处理大大增加了酶抗性，1%表没食子儿茶素没食子酸酯提供的保护相当于1%戊二醛。高达0.5 mM的EGCG对包埋在藻酸盐中的软骨细胞基本上没有细胞毒性，并且附着于脱细胞的、EGCG固定的软骨的自体软骨细胞在接种后5天都是存活的。结果表明，EGCG对脱细胞骨软骨异种移植物具有许多有益的作用，并且可以适用于在植入以修复缺损之前稳定这种移植物。
As a non-crosslinked osteochondral xenograft would be mechanically inferior to native cartilage and vulnerable to premature degradation, we seek a safe and effective method of xenograft stabilization. The purpose of this study was to evaluate the capacity for epigallocatechin gallate (EGCG) to stabilize a decellularized porcine osteochondral xenograft through collagen crosslinking. Our objectives were to assess the effects of EGCG on the degree of crosslinking, mechanical properties, collagenase resistance, cytotoxicity, and in vitro biocompatibility. EGCG is a green tea polyphenol that acts as a collagen crosslinker. Porcine osteochondral plugs were decellularized and then crosslinked by soaking in EGCG. The degree of crosslinking, cartilage compressive stiffness, cartilage-bone interface strength, coefficient of friction, and residual mass after collagenase exposure all increased with an increasing EGCG concentration. With the exception of the coefficient of friction, EGCG treatment could restore mechanical properties to levels equal to, or exceeding those, of native cartilage. EGCG treatment profoundly increased the enzymatic resistance, and 1% EGCG provided protection equivalent to 1% glutaraldehyde. EGCG up to 0.5 mM was essentially not cytotoxic to chondrocytes embedded in alginate, and autologous chondrocytes attached to decellularized, EGCG-fixed cartilage were all viable five days after seeding. Results demonstrate that EGCG has many beneficial effects on a decellularized osteochondral xenograft, and may be suitable for use in stabilizing such a graft prior to implantation for the repair of a defect.
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影响因子：
6.7
作者：
Ma B;Wang X;Wu C;Chang J
通讯作者：
Chang J
影响因子：
4.9
作者：
Englert C;Blunk T;Müller R;von Glasser SS;Baumer J;Fierlbeck J;Heid IM;Nerlich M;Hammer J
通讯作者：
Hammer J
影响因子：
3.7
作者：
Natarajan V;Madhan B;Tiku ML
通讯作者：
Tiku ML
影响因子：
4.8
作者：
Farr, Jack;Gracitelli, Guilherme C.;Gomoll, Andreas H.
通讯作者：
Gomoll, Andreas H.
影响因子：
2.8
作者：
Elder, Steven;Pinheiro, Amanda;Wright, Emily
通讯作者：
Wright, Emily