Clinical and experimental studies regarding the expression and diagnostic value of carcinoembryonic antigen-related cell adhesion molecule 1 in non-small-cell lung cancer.
Clinical and experimental studies regarding the expression and diagnostic value of carcinoembryonic antigen-related cell adhesion molecule 1 in non-small-cell lung cancer.
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癌胚抗原相关细胞粘附分子1在非小细胞肺癌中的表达及诊断价值的临床及实验研究
DOI：
10.1186/1471-2407-13-359
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发表时间：
2013-07-25
期刊：
影响因子：
3.8
通讯作者：
Gao F
中科院分区：
文献类型：
--
作者：
Zhou MQ;Du Y;Liu YW;Wang YZ;He YQ;Yang CX;Wang WJ;Gao F
癌胚抗原相关细胞黏附分子1（CEACAM1）是一种多功能的免疫球蛋白样细胞黏附分子，具有广泛的生物学功能。据先前报道，血清CEACAM1在不同恶性肿瘤中表达失调，并与肿瘤进展相关。然而，非小细胞肺癌（NSCLC）患者血清CEACAM1的表达情况尚不清楚。在NSCLC中，CEACAM1 - S和CEACAM1 - L异构体的不同表达比例也鲜有研究。本研究旨在探讨NSCLC患者血清CEACAM1水平以及CEACAM1 - S/L异构体的比例。
采用酶联免疫吸附试验检测血清CEACAM1的表达。通过免疫组织化学染色观察肿瘤组织中CEACAM1的蛋白表达及定位。运用实时荧光定量聚合酶链反应（qRT - PCR）检测肿瘤及癌旁正常组织中CEACAM1的信使核糖核酸（mRNA）水平，并通过逆转录聚合酶链反应（RT - PCR）分析CEACAM1 - S和CEACAM1 - L的表达模式及比率。
NSCLC患者血清CEACAM1水平显著高于正常健康对照者（P < 0.0001）。21例患者中17例（81%）免疫组织化学染色显示CEACAM1高表达。尽管肿瘤组织与正常组织中CEACAM1的mRNA表达水平无显著差异（P > 0.05），但肿瘤组织中CEACAM1 - S及CEACAM1 - S/L（S:L）比率显著高于正常组织（P < 0.05）。
我们的数据表明，血清CEACAM1水平可区分肺癌患者与健康人群，CEACAM1可能是NSCLC早期诊断的有用标志物。此外，我们的结果显示，即使肿瘤组织中总CEACAM1无显著变化，CEACAM1异构体的表达模式在肿瘤发生过程中也可能改变。我们的研究提示，CEACAM1 - S/CEACAM1 - L的表达比率在NSCLC诊断中可能比CEACAM1的定量变化更具指示意义。
BackgroundCarcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM1) is a multifunctional Ig-like cell adhesion molecule that has a wide range of biological functions. According to previous reports, serum CEACAM1 is dysregulated in different malignant tumours and associated with tumour progression. However, the serum CEACAM1 expression in non-small-cell lung carcinomas (NSCLC) is unclear. The different expression ratio of CEACAM1-S and CEACAM1-L isoform has seldom been investigated in NSCLC. This research is intended to study the serum CEACAM1 and the ratio of CEACAM1-S/L isoforms in NSCLC.MethodsThe expression of the serum CEACAM1 was determined by enzyme-linked immunosorbent assay. The protein expression and the location of CEACAM1 in tumours were observed by immunohistochemical staining. The CEACAM1 mRNA levels in tumour and normal adjacent tissues were measured using quantitative real-time PCR, and the expression patterns and the rate of CEACAM1-S and CEACAM1-L were analysed by reverse transcription-PCR.ResultsSerum CEACAM1 levels were significantly higher in NSCLC patients compared with that from normal healthy controls (P<0.0001). 17 patients (81%) among 21 showed high expression of CEACAM1 by immunohistochemical staining. Although no significant differences were found between tumour and normal tissues on mRNA expression levels of CEACAM1 (P>0.05), the CEACAM1-S and the CEACAM1-S/L (S: L) ratios were significantly higher in tumour than normal tissues (P<0.05).ConclusionsOur data indicated that the serum levels of CEACAM1 could discriminate lung cancer patients from health donors and that CEACAM1 might be a useful marker in early diagnosis of NSCLC. Moreover, our results showed that the expression patterns of CEACAM1 isoforms could be changed during oncogenesis, even when total CEACAM1 in tumour tissues did not show significant changes. Our study suggested that the expression ratios of CEACAM1-S/CEACAM1-L might be a better diagnostic indicator in NSCLC than the quantitative changes of CEACAM1.
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DOI：
10.1165/ajrcmb.11.2.8049082
发表时间：
1994-08-01
影响因子：
6.4
作者：
OHWADA, A;TAKAHASHI, H;KIRA, S
通讯作者：
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影响因子：
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作者：
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作者：
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作者：
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作者：
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