Insight into the specific virulence related genes and toxin-antitoxin virulent pathogenicity islands in swine streptococcosis pathogen Streptococcus equi ssp. zooepidemicus strain ATCC35246.
Insight into the specific virulence related genes and toxin-antitoxin virulent pathogenicity islands in swine streptococcosis pathogen Streptococcus equi ssp. zooepidemicus strain ATCC35246.
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深入了解猪链球菌病原体链球菌等猪链球菌中的特定毒力相关基因和毒素 - 抗毒素的致病岛。 ZOOEPIDEMICUS菌株ATCC35246。
DOI：
10.1186/1471-2164-14-377
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发表时间：
2013-06-07
期刊：
影响因子：
4.4
通讯作者：
Hu S
中科院分区：
文献类型：
--
作者：
Ma Z;Geng J;Yi L;Xu B;Jia R;Li Y;Meng Q;Fan H;Hu S
马链球菌亚种zooprophylmicus（S.是引起我国猪链球菌病的重要病原。致病性岛（PAI）。动物寄生虫通过水平基因转移（HGT）在细菌间转移，在沙门氏菌的适应和毒力增强中发挥重要作用。小动物。本研究采用比较基因组学方法对不同致病性的沙门氏菌进行了研究。小动物。S. ZooodermicusATCC 35246（Sz 35246）含有2，167，264-bp的单环状染色体，GC含量为41.65%。Sz35246、S.动物链球菌MGCS 10565（Sz 10565）、马链球菌（Streptococcus equi.）SSP.等。4047（Se 4047）和S. Zooplasticmicus H70（Sz 70）共鉴定出320个Sz 35246特异性基因，分为3个毒素-抗毒素（TA）系统PAI和1个限制性修饰系统（RM系统）派。这四种获得性PAI编码的蛋白质可能有助于该细菌适应不同宿主的整体致病能力和适应性。对该细菌的体内和体外转录组的分析揭示了差异表达的派基因和非PAI基因，表明Sz 35246具有感染动物和适应广泛宿主环境的机制。基因组分析确定了潜在的Sz 35246毒力基因。推测Fim III操纵子的基因参与打破Sz 35246的宿主限制。Sz 35246与其他三个菌株的全基因组比较和转录组分析揭示了与细菌毒力和打破宿主限制相关的新基因。首次鉴定出4种特异性PAI，经HGT鉴定，推测其已转移到Sz 35246基因组中。对PAI中TA和RM系统的进一步分析将提高我们对该细菌致病性的理解，并可能导致诊断和疫苗的开发。
Streptococcus equi ssp. zooepidemicus (S. zooepidemicus) is an important pathogen causing swine streptococcosis in China. Pathogenicity islands (PAIs) of S. zooepidemicus have been transferred among bacteria through horizontal gene transfer (HGT) and play important roles in the adaptation and increased virulence of S. zooepidemicus. The present study used comparative genomics to examine the different pathogenicities of S. zooepidemicus. Genome of S. zooepidemicus ATCC35246 (Sz35246) comprises 2,167,264-bp of a single circular chromosome, with a GC content of 41.65%. Comparative genome analysis of Sz35246, S. zooepidemicus MGCS10565 (Sz10565), Streptococcus equi. ssp. equi. 4047 (Se4047) and S. zooepidemicus H70 (Sz70) identified 320 Sz35246-specific genes, clustered into three toxin-antitoxin (TA) systems PAIs and one restriction modification system (RM system) PAI. These four acquired PAIs encode proteins that may contribute to the overall pathogenic capacity and fitness of this bacterium to adapt to different hosts. Analysis of the in vivo and in vitro transcriptomes of this bacterium revealed differentially expressed PAI genes and non-PAI genes, suggesting that Sz35246 possess mechanisms for infecting animals and adapting to a wide range of host environments. Analysis of the genome identified potential Sz35246 virulence genes. Genes of the Fim III operon were presumed to be involved in breaking the host-restriction of Sz35246. Genome wide comparisons of Sz35246 with three other strains and transcriptome analysis revealed novel genes related to bacterial virulence and breaking the host-restriction. Four specific PAIs, which were judged to have been transferred into Sz35246 genome through HGT, were identified for the first time. Further analysis of the TA and RM systems in the PAIs will improve our understanding of the pathogenicity of this bacterium and could lead to the development of diagnostics and vaccines.
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影响因子：
3.7
作者：
Chen C;Tang J;Dong W;Wang C;Feng Y;Wang J;Zheng F;Pan X;Liu D;Li M;Song Y;Zhu X;Sun H;Feng T;Guo Z;Ju A;Ge J;Dong Y;Sun W;Jiang Y;Wang J;Yan J;Yang H;Wang X;Gao GF;Yang R;Wang J;Yu J
通讯作者：
Yu J
影响因子：
5.5
作者：
Fan Hong-jie;Tang Fu-yu;Lu Cheng-ping
通讯作者：
Lu Cheng-ping
DOI：
10.1099/00221287-143-2-553
发表时间：
1997-02-01
期刊：
MICROBIOLOGY-UK
影响因子：
--
作者：
Bloomfield, GA;Whittle, G;Cheetham, BF
通讯作者：
Cheetham, BF
影响因子：
5.1
作者：
Bolhuis, H;Poele, EMT;Rodriguez-Valera, F
通讯作者：
Rodriguez-Valera, F
方形古夸夸氏菌的基因组walsbyi：水活动极限的生命。
影响因子：
4.4
作者：
Bolhuis, Henk;Palm, Peter;Wende, Andy;Falb, Michaela;Rampp, Markus;Rodriguez-Valera, Francisco;Pfeiffer, Friedhelm;Oesterhelt, Dieter
通讯作者：
Oesterhelt, Dieter